Day 1 :
Keynote Forum
Tom Jobe
Chief Operating Officer at SensiQ Technologies, USA
Keynote: New SPR-based biosensor platform for fragment-based-drug-discovery
Time : 10:00-10:25
Biography:
Tom Jobe holds BS and MS Engineering degrees from Oklahoma State University. He spent 11 years in the energy business as an instrument developer and 25 years as a developer and manager of medical diagnostic instruments with Organon Teknika & BioMerieux, where he led the development of microbiology, coagulation, and nucleic acid diagnostics instrumentation. He currently is the Chief Operating Officer of SensiQ Technologies and has directed the R&D and Manufacturing operations for the company’s life sciences instrumentation and commercialization efforts for the past 10 years.
Abstract:
The detection and characterization of fragment binding events is dependent upon sensitive biophysical technologies capable of detecting low affinity interactions of low molecular weight compounds. Current Surface-Plasmon-Resonance based biosensors are limited in the processing and subsequent data analyses of fragment binding events. A new Surface Plasmon Resonance –based platform has been developed to specifically address binding analysis requirements in fragment screening. New injection techniques and data analysis methods illustrating rapid and automated hit selection capabilities will be presented. At the heart of the new platform is a new gradient concentration technique that allows affinity values to be obtained with single injections. Rapid hit selection identification is performed using a new statistical analysis method which allows data analysis to be competed in minutes. Relevant industry case studies will be highlighted to demonstrate fragment screening workflow optimization. The new platform potentially offers researchers a significant advancement in workflow and minimized assay development substantially over conventional SPR platforms used in fragment-based-drug-discovery.
Keynote Forum
David C Jiles
Palmer Department Chair in Electrical and Computer Engineering, Iowa State University, USA
Keynote: Deep transcranial magnetic stimulation for the treatment of neurological disorders
Time : 10:25-10:50
Biography:
David Jiles has worked on magnetics for 35 years, authored more than 600 scientific papers, have published three books, and hold 19 patents. He has served as Editor-in-Chief of IEEE Transactions on Magnetics since 2005-2012. His research interests are in applications of magnetics including biomedical applications. His lab has developed new coil configurations called the “Halo Coil†that can stimulate deeper regions with low focality or cortical regions with high focality. He is a fellow of Royal Academy of Engineering, Institute of Electrical and Electronics Engineers, the American Physical Society, the Institute of Physics and the Institute of Materials.
Abstract:
Transcranial magnetic stimulation (TMS) is a non-invasive, surgery free and safe neuromodulation technique that uses time varying magnetic fields to alter the functions of a targeted brain region. TMS has been FDA approved for the treatment of major depressive disorder by stimulating the left dorsolateral pre-frontal. Many neurological disorders such as Parkinson’s disease, Post-Traumatic Stress Disorder originate from the deeper parts of the brain. With increase in median age of the population, the prevalence of neurodegenerative diseases is becoming common. In order to treat these disorders non-invasively, deep TMS is critically needed. Magnetic field generated from the TMS coil decays rapidly with distance from the surface of the coil. This makes it challenging to apply stronger magnetic fields at deeper regions of the brain. We have developed novel family of coil configurations called the “Halo Coilâ€and“Triple Halo Coil†which increase the fields in the deeper regions of the brain by reducing the decay rate from the field coil placed on top of the head. The first generation “Halo Coil†can induce a field of 3 times higher at 10cm depth than the single circular coil. The second generation “Triple Halo Coil†can generate a field of 12 times higher than the circular coil at a depth of 15cm. We have used an anatomically realistic head model considering different electrical and magnetic properties of tissues for the determination of electric and magnetic fields inside the brain. We have verified our calculation by magnetic field measurements around the TMS coils.
Keynote Forum
Thomas F Floyd
Interim Division Chief of Cardiac Anesthesia, Stony Brook University, USA
Keynote: Spinal fiber optic monitoring
Time : 11:05-11:30
Biography:
Floyd obtained his MD from the University of Pennsylvania in 1986 and completed his residency in Anesthesiology in 1990 at the University of Minnesota. He completed a fellowship in Magnetic Resonance Imaging as well as a fellowship in Cardiothoracic Anesthesiology from 1999-2002 at the University of Pennsylvania where he subsequently served as a faculty. He currently is a Professor with Tenure in the Department of Anesthesiology and maintains adjunct appointments in Biomedical Engineering, Neurology, and Radiology at Stony Brook University in New York. He is a lead investigator in the development of fiber optics for the monitoring of spinal cord ischemia.
Abstract:
Spinal cord ischemia results in life-changing paralysis and paraparesis after major vascular, spine and spinal cord surgery, and spine trauma. No technology is currently available to directly and immeadiately predict or detect the onset of spinal cord ischemia, nor provide feedback and guidance for interventions directed at improving flow and oxygen delivery to resolve the ischemia. Current methods employed to detect spinal cord ischemia, based upon electrophysiology, are indirect, temporally insensitive, nonspecific, as only 16%–40% of patients with electrophysiological changes developed postoperative-onset paraparesis, paraplegia, or quadriplegia. The reliability of the current technology is simply not good enough. We have developed a prototypical fiber optic device based on Diffuse Correlation Spectroscopy (DCS) and Diffuse Optical Spectroscopy (DOS) principles that allows for the immediate detection and continuous monitoring of changes in spinal cord blood flow and oxygenation. The device prototype can be placed via open and percutaneous approaches. The ability to measure spinal cord blood flow and oxygenation will: 1) facilitate expeditious diagnosis and monitoring of the progress of spinal cord ischemia; 2) enable continuous bedside monitoring in the neurocritical care setting; 3) offer an enhanced opportunity to prevent secondary injury; 4) provide critical data in the laboratory and clinic for assessment of the efficacy of therapeutic approaches to ameliorate ischemia, and 5) provide preoperative and intraoperative data to improve decision making for procedures where the spinal cord may be threatened.
Keynote Forum
Mark A Reed
Associate Director of the Yale Institute for Nanoscience and Quantum Engineering, Yale University, USA
Keynote: Electronic label - free biosensing assays
Time : 11:30-11:55
Biography:
Mark A Reed holds the Harold Hodgkinson Chair of Engineering and Applied Science at Yale University, and is the Associate Director of the Yale Institute for Nanoscience and Quantum Engineering. He is the author of more than 180 professional publications and 6 books, has given over 25 plenary and over 360 invited talks. His awards include the Kilby Young Innovator Award, the Fujitsu ISCS Quantum Device Award, Fellow of the American Physical Society, Fellow of the Institute of Electrical and Electronics Engineers, and the IEEE Pioneer Award in Nanotechnology.
Abstract:
Nanoscale electronic devices have the potential to achieve exquisite sensitivity as sensors for the direct detection of molecular interactions, thereby decreasing diagnostics costs and enabling previously impossible sensing in disparate field environments. Semiconducting nanowirefield effect transistors (NW-FETs) hold particular promise, though contemporary NW approaches are inadequate for realistic applications and integrated assays. We present here an integrated nanodevice biosensor approach that is compatible with CMOS technology, has achieved unprecedented sensitivity, and simultaneously facilitates system-scale integration of nanosensors. These approaches enable a wide range of label-free biochemical and macromolecule sensing applications, such as specific protein and complementary DNA recognition assays, and specific macromolecule interactions at femtomolar concentrations. Critical limitations of nanowire sensors are the Debye screening limitation, and the lack of internal calibration for analyte quantification, which has prevented their use in clinical applications and physiologically relevant solutions. We will present approaches that solve these longstanding problems, which demonstrate the detection at clinically important concentrations of biomarkers from whole blood samples, integrated assays of cancer biomarkers, and the use of these as a quantitative tool for drug design and discovery, including binding kinetics and chirality detection.
Keynote Forum
Anis Rahman
President/CTO at Applied Research & Photonics Inc., USA
Keynote: Terahertz spectral profiling and imaging for skin cancer detection
Time : 11:55-12:20
Biography:
Anis Rahman is known for his work on Dendrimer based photonics and terahertz technology. He Coined the term “silicon for photonics†and his approach makes it possible to fabricate chip based components from dendrimer for sensing and terahertz generation. He proposed a new mechanism, dendrimer dipole excitation, that generated continuous wave terahertz over a broad spectrum. Under his leadership, dendrimer technology received prestigious awards including the NASA Nanotech Brief’s nano-50 award and CLEO/Laser Focus World’s Innovation award (2011). He completed MS & PhD at Marquette University (Milwaukee, WI) and a postdoctoral research position at Columbia University (NY).
Abstract:
Terahertz scanning reflectometry enables investigating both the surface and the sub-surface of biological tissues in non-invasive fashion. T-ray is non-ionizing, thus, eliminates radiation damage of sensitive tissues while still probing disease conditions in the deeper layers leading to an effective early diagnostic tool. In this study, terahertz techniques have been developed that is comprised of terahertz scanning reflectometry, terahertz time-domain spectroscopy and terahertz 3D imaging (all instruments from Applied Research & Photonics, Harrisburg, PA 17111) for detection of basal cell carcinoma (BCC) in comparison with the benign skin samples. Benign skin biopsy samples and the biopsy from cancerous area were investigated. Thickness profiling exhibits significant differences in profiles of the respective skin samples both in their layer structure and also in their total reflected intensities; thus indicating presence and lack of cellular order for the respective specimens. Terahertz spectra, acquired in transmission, exhibit quantifiable differences for both groups. Additionally, 3D terahertz image of the benign skin shows regular cell patterns while the images of BCC sample exhibit irregular and agglomerated cell patterns. The lack of cellular order in the skin, thus, may be used as an indication of cancer forming process. This finding, therefore, may be used as an early diagnostic tool. It is notable that this is the first of such a concerted observation of benign versus BCC skin samples from three different experiments. The results are consistent from individual experiments and collectively provide an accurate means of early detection of BCC.
- Track 1: Biosensors
Track 2: Biosensors Applications
Track 4: Bioelectronics
Track 10: Advancement in Nanotechnology
Session Introduction
Yu Lei
University of Connecticut, USA
Title: PEG-BSA-Coumarin-GOx fluorescent hydrogel: Preparation, characterization and glucose biosensing
Time : 12:20-12:40
Biography:
Yu Lei is a Castleman Associate Professor of Chemical and Biomolecular Engineering and Biomedical Engineering at the University of Connecticut, USA. He obtained his PhD degree in 2004 at the University of California-Riverside in Chemical and Environmental Engineering. His current research combines biotechnology, nanotechnology, and sensing technology, especially as applied to the development of gas sensors, electrochemical sensors, and biosensors.
Abstract:
Diabetes mellitus is a chronic metabolic disorder caused due to lack of insulin production by pancreatic islet cells (Type I) or underutilization of insulin produced in the pancreas (Type II). Complications arising due to this disease can be minimized by vigilant monitoring of blood glucose levels. In this study, a fluorescent enzymatic hydrogel was designed for rapid glucose detection. The formation of glucose-responsive hydrogen was realized using crosslinking of functional poly (ethylene glycol), protein, glucose oxidase (GOx) and Coumarin-NH2, in which GOx serves as glucose-recognition element and pH-sensitive Coumarin-NH2 as a fluorescence “turn-on†reporter. Thermal and mechanical properties of the hydrogel scaffold with and without sensing elements were systematically analyzed. Especially the compression analysis of the fluorescent hydrogel shows high elasticity with good mechanical strength. The as-developed fluorescent hydrogel shows fast response time, good sensitivity and good reproducibility at physiological pH upon the addition of glucose. The glucose sensing mechanism was relied on that GOx takes glucose as substrate to liberate proton, which results in the fluorescence “turn-on†of pH-sensitive Courmarin-NH2 covalently immobilized in the hydrogel. The developed fluorescence hydrogel holds great promise as an injectable glucose biosensor for in-vivo continuous glucose monitoring.
Laurent A Francis
Universite Catholique de Louvain, Belgium
Title: The development of integrated capacitive array biosensors towards the selective and real-time detection of single bacterium
Time : 12:40-13:00
Biography:
Laurent A Francis received MS and PhD degrees from the Université Catholique de Louvain (UCL), Belgium, in 2001 and 2006, respectively. He is currently working as an Associate Professor at UCL. His research interests are related to co-integrated, ultra-low power CMOS MEMS sensors for biomedical applications and harsh environments. He was previously Researcher at IMEC in Leuven, Belgium, and a visiting Professor at the Université de Sherbrooke, Canada. He is author or co-author of more than 70 scientific articles, has co-edited one book and holds one patent. He is member of the Belgian National Committee on Biomedical Engineering and of the IEEE.
Abstract:
The rapid and selective detection of whole bacterial cells is of interest for the development of Point-of-Care (PoC) diagnosis tools and Lab-on-Chip (LoC) systems. Different types of transducers are promising to that end, including in particular capacitive biosensors. For them, we present here a three-step approach to investigate the AC impedance spectroscopy of interdigitated microelectrodes coated by an ultrathin passivation layer and operated in electrolytes with bacterial cells adherent to the surface. The first step is an accurate quantification of the device sensitivity helped by analytical models and two-dimensional simulations. The simulations are based on Poisson-Nernst-Planck equations and include the modeling of the sensor topology, the dielectric properties of multi-shell bacteria, the various ionic transports, and surface and space charges. The second step is to ensure the selectivity by involving lysostaphin for genus-specific bacterial detection. The sample matrix is then directly flown on the polydopamine-covered sensor surface without any pre-treatment. The third step is the integration of microelectrodes with a 0.25 µm CMOS process as the pixel element of a capacitive array. A capacitive-to-voltage conversion was designed below each pixel and the sensitivity is boosted by a subthreshold gain stage. The capacitive biosensor array performs dielectric measurement of the electrolytes and, as proof of concept, the real-time detection of Staphylococcus epidermidis binding events with a detection limit of about 5 bacteria per pixel. Pushing further the limits of the device will drive to small footprint and low power consumption capacitive array for the real-time detection of single bacterium.
Peng Chen
Nanyang Technological University, Singapore
Title: Nanoelectronic detection of dynamic Cell functions
Biography:
Peng Chen is currently an Associate Professor and Program Director of Bioengineering in School of Chemical and Biomedical Engineering at Nanyang Technological University (Singapore). He completed his PhD at University of Missouri (Columbia) in 2002. This was followed by a period of Post doctoral Research at Harvard University. His research interests are in the areas of nanomaterials, biosensors, and bio nanotechnology, which has led to >130 publications in leading journals. He is particularly interested in applying interdisciplinary and integrative approaches to study biomedical problems.
Abstract:
As the current follows solely or largely on the surface, the conductance of the semiconducting nanomaterials is highly sensitive to the electrochemical perturbation induced by the interacting biomolecules or by biological activities of the interacting cells. Taking advantage of this, nanoelectronic biosensors based on these nanostructured materials have been developed, promising novel applications for fundamental studies, diagnosis, and drug screening. This presentation briefly reviews our works on the nanoelectronics-biology interface. More specifically, we have developed and used nanoelectronic field-effect transistors based on carbon nanotubes, graphene, or silicon nanowires to electrically detect the presence of biomolecules or dynamic activities of live cells (secretion of biomolecules or chemicals, ion channel activities, metabolic activities), with high sensitivity, high temporal resolution, and high throughput.
Gymama Slaughter
University of Maryland, USA
Title: Recent Development in Power Systems for Implantable Bioelectronic Devices
Time : 14:00-14:20
Biography:
Gymama Slaughter received her B.S. in Chemistry and her Ph.D. in Computer Engineering from the Virginia Commonwealth University. She was the Director of the Center for Biosystems and Engineering at Virginia State University. She joined the Computer Science and Electrical Engineering Department at the University of Maryland Baltimore County in 2010. Prof. Slaughter is the Director of the Bioelectronics Laboratory and is a recipient of the NSF CAREER award in 2014. Her work is on the design and development of biosensor and nanoelectrode arrays for monitoring blood glucose and neurological disorders. She has published 24 peer-reviewed scientific articles on her work.
Abstract:
Microfabrication is an enabling technology with a broad set of applications that continues to grow. One major challenge in implementing bio-implantable bioelectronic devices such as continuous glucose monitor is to develop it so that it can power itself. We develop miniaturized integrated self-powered biosensing microsystems that is simple yet fully functional to conduct frequent, autonomous glucose sensing experiments in vitro and in vivo. In our laboratory, we are exploring the design, fabrication, and testing of flexible electrochemical power systems with integrated glucose biosensing capability. The multi-functional device will not only have the ability to generate bioelectricity from the direct conversion of the chemical energy stored in glucose, it will also utilize the energy generated by the transduction of chemical energy into electrical energy resulting from glucose as the fuel source to determine the concentration of glucose. One can envision implanting such a device into patients with diabetes thereby harnessing bioelectricity from within the body to power ultra-low power bioelectronic devices and obtaining blood glucose measurement simultaneously. This talk will highlight the importance of electrochemical power systems to medicine and how they can be employed to harness the biochemical energy from within the body.
Nicole McFarlane
University of Tennessee, USA
Title: Vertically aligned carbon nanofiber biosensors
Time : 14:20-14:40
Biography:
Nicole McFarlane received the BS and MS degrees in electrical engineering from Howard University, Washington DC, in 2001 and 2003 respectively, and her PhD in Electrical Engineering at the University of Maryland, College Park in 2010. Her research experience includes growing and characterizing III–V Nitrides, understanding information and power efficiency trade-offs in mixed signal integrated circuit design, CMOS biosensors and CMOS/MEMS integration for lab-on-a-chip technologies. She has been an Assistant Professor at the University of Tennessee since 2010, working on sensors, devices and electronics for portable and implantable applications.
Abstract:
Electrophysiological, electrochemical and electroanalytical detection performed using electrodes in the nanometer range have emerged as promising avenues for the interrogation and monitoring of real time biochemical dynamics at the single cell level. The promise of these electrodes lie in their fast response times, high mass sensitivity, small size, large linear dynamic range, and molecules of interest which can be followed without the need for chemical derivitization, as is necessary with fluorescent probe techniques. In the large variety of sensor materials available, carbon is a popular sensing electrode due to its unique structural and material properties. These properties include high conductivity, durability in harsh environment, and inertness to processing steps. The crucial advantage of aligned carbon nanofibers over other nanostructures, such an carbon nanotubes, is that they can be grown deterministically such that their position, height, tip diameter, and, to some extent, shape and orientation can all be controlled. Additionally, reliable mechanical and electrical contact to the substrate can be established because of their excellent conductive and structural properties. In collaboration with Syed Islam’s group and Oak Ridge National Lab, we have demonstrated an electrochemical biosensing platform using vertically aligned carbon nanofibers. The sensor is capable of sensing a wide range of physiolocially relevant glucose levels and has shown excellent repeatablility, linearity, sensitivity and resolution. This talk will present the status of our work including our experimental results and future plans for the technology.
Muhammad Mujeeb-U-Rahman
Wireless Diagnostic Systems, Inc, USA
Title: Novel miniaturized, fully integrated, Wireless, Low-cost Glucose Sensing Platform
Time : 14:40-15:00
Biography:
Muhammad Mujeeb-U-Rahman did his MS and PhD in Electrical Engineering at Caltech. His thesis work was focussed on developeing novel fully integrated systems which can perate as wireless sensors in complex biochemical environment like human body. This work has lead to the inception of a funded startup company focused on developing next generation glucose sensor products. Dr. Mujeeb-U-Rahman received the Demetriades - Tsafka - Kokkalis Prize for best work in Nanotechnology at Caltech in 2014. He is also a Fulbright fellow for his MS. Prior to coming to Caltech, he was founding member of the WiMAX research group at Caltech which was started based upon his senior thesis work.
Abstract:
Diabetes is a significant health care issue which affects millions of people worldwide. Diabetes management at different stages requires glucose sensing followed by appropriate medical actions. Accurate and reliable sensing is an important component for the complete solution to disease management as well as to scientific studies required for better understanding of the disease and effect of different therapeutic factors for its prevention or treatment. To achieve the goal of low-cost, long term solution for accurate glucose sensing, we have been working on developing a novel, fully-integrated sensing technology which holds the key to solve the significant challenges inherent in current glucose sensing technologies. This system is based upon CMOS integrated electrochemical sensors with integrated control and autonomous wireless operation. A combination of extremely efficient circuit design, wireless operation (using RFID like technology) and Nanotechnology allows extreme miniaturization resulting in the smallest working platform to date. I will also present a smart external reader technology that can be used as a wearable device for continuous reading or used with smart phone for discrete readouts. In this talk, I will go through the motivation behind our smart design and the advantages it has over current devices, both commercial and research devices. I will explain the key design challenges and their effect on the overall system design. I will present the status of our work, including extensive in-vitro and preliminary in-vivo results. I will conclude with the plan to move forward with this technology and the clinical strategy to get this technology ready for human use.
Shen Ming Chen
National Taipei University of Technology, Taiwan
Title: Eco-friendly syntheses of advanced carbon-based nanomaterials for electrochemical biosensors and energy storage applications
Biography:
Will get updated shortly....!
Abstract:
The existing carbon materials can be classified into activated carbon (0-dimensional), carbon nanotubes (CNT) (1-dimensional), graphene (2-dimensional) and carbon foams (3-dimensional). Among these, graphene is well known to be the top candidate; However, preparation of graphene from graphite is an intricate procedure that can lead to an explosion during the oxidation of graphite. Similarly, the preparation of CNT also has some practical difficulties due to the complicated instrument setup. Fascinatingly, the preparation of ACs is simple, environmentally friendly and cost-effective. For the first time, activated carbon (ACs) with high surface area and volume has prepared by ZnCl2 activation at three different temperatures (700-900°C), using a simple and eco-friendly method. The morphology and chemical composition of the resultant ACs were characterized by field emission scanning electron microscopy, high-resolution transmission electron microscopy, elemental analysis, X-ray photoelectron spectroscopy and Raman spectroscopy. Here, we used the AC-700°C (AC) modified glassy carbon electrode (GCE) for the simultaneous and selective electrochemical detection of AA, DA and UA and dye-sensitized solar cell applications. Furthermore, a novel spherical carbon nanoparticle decorated activated carbon (SNAC) material with a high surface area of about 1555 m2g-1 is prepared from dead mango leaves by an eco-friendly method for the detection of toxic heavy metal ions and energy storage applications. The amounts of carbon, hydrogen, nitrogen and sulfur in SNAC are determined to be 72.6, 6.1, 6.5, and 7.46%, respectively. The limits of detection (LODs) for the determination of Cd(II), Pb(II), Cu(II), and Hg(II) ions at the SNAC-modified GCE are 24.4×10-9 M, 5.7×10-9 M, 23.2×10-9 M and 24.6×10-9 M, respectively. On the other hand, the maximum obtained specific capacitance is 478 F g-1 at a scan rate of 5 mV s-1. In addition, we prepared a novel nanocomposite viz. gold nanoparticles (Au) decorated activated carbon (SNAC) modified glassy carbon electrode by a simple electrochemical approach to trace level detection of hydrazine. The amounts of carbon, hydrogen, nitrogen and oxygen functional groups is a promising way to hold the Au nanoparticles in the carbon network. The electro-oxidation of hydrazine has been assessed by cyclic voltammetry, linear sweep voltammetry and amperometric sensor. Furthermore, the high-surface-area (~1456 m2 g-1) with highly porous and heteroatoms-enriched in nature of activated carbon (HAC) was prepared from the banana stem (Musa paradisiaca Family:Musaceae) at different carbonization temperatures of 700ºC, 800ºC and 900ºC. The HAC contains the amount of carbon, hydrogen, nitrogen and sulfur are determined to be 61.12, 2.567, 0.4315, and 0.349%, respectively. Moreover, we prepared a novel nanocomposite viz. nickel oxide (NiO) decorated activated carbon (HAC) modified glassy carbon electrode by a simple electrochemical approach for a novel non-enzymatic glucose sensor. The oxygen functional groups are a promising way to hold the NiO nanoparticles in the carbon network. The fabricated dye sensitized solar cells (DSSCs) with Pumpkin stem-AC counter electrodes shown the higher power conversion efficiency of 2.79%.
Hossam Sayour
Animal Health Research Institute, Egypt
Title: Biosensors technology and Molecular Imprinted Polymers: Potential Applications of Theranostics, Food safety and Environment.
Biography:
Hossam Sayour has completed his PhD at the age of 36 years from Ain Shams University and postdoctoral studies from Animal Health. He is leader of research group of sensors and biosensors, a mentor of emerging molecular imprinting applications in theranostics, food safety and environment. He has published more than 17 papers in reputed journals and has been serving as multidisiplinary research team advisor in both biomedical, environmental engineering.
Abstract:
No society is capable of reaching the goals of a comprehensive, sustainable development, and facing the demands of the future except with knowledge, technological development and innovation, through channeled academic research, which is considered in any society the cornerstone to academic progress and development. Therefore, advanced countries strove to increase their support of academic research and its development. Methods and multiple forms were founded to achieve the utmost avail of academic research in its various types with special attention to the innovative, technological and industrial side. As a result, there emerges an escalation in endowing chaired positions in advanced and future sciences, expanding the establishment of research and technology parks, incubators, centers for innovation and excellence, the valleys of research and development, all are efforts aiming at initiating fertile domains for innovation. Importance of effecting more interconnection and integration among the functions and activities of science and technology institutions, along with creativity Biosensors Technology and Molecular Imprinted Polymers: Potential Applications of Theranostics, Food Safety and Environment was suggested. Early identification and rapid investigation are crucial for outbreak prevention. Several international organizations such as WHO, OIE, FAO and EPA called upon the development of rapid, sensitive, low cost, and easy to use early diagnosis of pathogens “rapid field test†or “point of care diagnosticsâ€. The detection and monitoring diseases has been a huge burden due to the high cost of reagents, laboratory sophisticated equipment s and trained personals. Most of expenses spent for disease diagnoses go to analytical and diagnostic devices. Moreover, laboratories are hard to find in the remote epidemic areas and agriculture farms. There has been tremendous development and advancements in the field of Molecular biology, Nanotechnology, Electromechanical, Bio-MEMS systems and wireless sensors networks (WSN’s). These advanced technologies led to the development of Bio-microchip devices for the detection of chemical and biological hazards. Lab-on-a-chip technique is considered to be one of the top emerging technologies. In chemistry, molecular imprinting is a technique to create template-shaped cavities in polymer matrices with memory of the template molecules the same merits of Lock and the Key theory/paradigm (Linus Pauling 1940). Scientists have been working for decades to mimic the exquisite molecular recognition ability of biological molecules such as antibodies, enzymes, and receptors. In the recent years, imprinted polymers have been used to capture/recognize everything from macromolecules to inorganic ions. Recognition plays an important role in biological systems and is observed in between receptor- ligand, antigen-antibody, DNA-protein, sugar-lectin, RNA-ribosome, substrate-enzyme etc. The world is living the plastic age so MIP’s would offer smart solutions in the fields of theranostics, food safety, environmental and sensor technology. Molecular Imprinted Polymers (MIPs) have been applied as artificial antibodies, catalysts, sensors, drug assay & delivery tools, and chromatographic separations. Finally, MIP is science goes market upon business analysis based on patents.
Ewelina Zabost
University of Warsaw, Poland
Title: Multicomponent biomaterials for improved storing, sensing and release
Biography:
Ewelina Zabost presently works as Associate Professor at University of Warsaw, Faculty of cheistry snd R&D support in High-Tech Companies. Responsible for managenement of National and International Research Projects (7), Mangement of Projects reffered to promotion and realization of innovative solutions at the workplace, teaching (preparation of several lectures and labs) and tutoring for Bachelor and Master studies at Faculty of Chemistry. Research activity in interdisciplinary fields of sciences: analytical chemistry, biophysics, biochemistry, pharmaceutical, drug delivery, biomaterials, nanomaterials, biosensors. Membership activity in 4 international scientific organizations (International Society of Electrochemistry, Bioelectrochemical Society, Royal Society of Chemistry, Controlled Release Society). Autor and co-autor of peer reviewed publications (10) and presentations (43). 2 golds, 1 silver medals and “Rising Star†title from Taiwan Prominent Inventor League for presenting of innovative solitions on International Fares. In board of audit and vice-president of two Associations in Poland: TOP 500 Innovators and Science-Management-Infrastructure-Support Association.
Abstract:
Recent interest in designing micro- and nano-sized drug delivery systems and layers is oriented on creation of delivery systems for poorly soluble and/or highly unstable substances. In our work we would like to expand the synthesis ways and optimization steps needed for preparation of multicomponent biomaterials with improved storage, delivery and sensing properties. Firstly, we would like to present steps involves the application of charge controlled coaxial core-shell electrospinning process for creation of thin micro- and nanocomposites of PLC/PNIPA/DNA/Au NPs with attached selected antitumor drugs. They were attached by noncovalent and covalent bonding sensitive to tumor environment. Modified gold nanoparticles were entrapped in PLCL fibers during electrospinning process. We investigated the release profiles of drug-modified Au NPs from PLC nanofibers, by spectroscopic (UV-Vis, CD) and electrochemical techniques (CV, SWV) and in vitro experiments (HeLa, Insulinoma and Glioma cells). The morphology of composites was inspected by TEM, SEM and optical microscopy. Secondly, we would like to show several kinds of metallic NPS and hydrogel based nanoparticles modified by selected aptamers. We applied PAM and PNIPA hydrogel networks, for formation of multicomponent nanoparticles for storing of selected antitumor intercalators and releasing of it after initiation of structural changes of aptamers and volume phase transition of lattices. The DNA-based biomaterials were characterized by a strong increase in guanine and adenine anodic currents that starts at physiological temperature. The structural alterations were used as a control element in the releasing of drugs.Thirdly, we would like to expand the possibility of application of multicomponent, metallic NPS and hydrogel-based nanoparticles modified covalently with selected aptamers as sensing layers used in electrochemical sensors. We optimized the design process of such biosensors for improved detection of MUC1 protein.
Wing-Cheung Law
The Hong Kong Polytechnic University, China
Title: Novel plasmonic sensing strategy based on semiconductor nanocrystals
Time : 15:00-15:20
Biography:
Wing-Cheung Law obtained his BE and MPhil degrees in Electronic Engineering from The Chinese University of Hong Kong in 2003 and 2005 respectively. He received PhD degree in Electrical Engineering from State University of New York at Buffalo, USA in 2011. Prior joining the Hong Kong Polytechnic University as an Assistant Professor in the Industrial and Systems Engineering, he has been serving as Research Assistant Professor in the Institute for Lasers, Photonics and Biophotonics of State University of New York at Buffalo, USA, conducting researches in the field of biophotonics and nanomedicine. His research focuses on building nanoparticle-based biosensing system and translating the bench-top setups into final products. He received two patents on designing surface plasmon resonance biosensors. He has published more than 80 peer reviewed journals and served as a reviewer for 20 international journals.
Abstract:
Nanoparticle-mediated sensing has become a major focus of research activity in recent years. The distinct features of the variety of nanoparticles have attracted significant attention in the chemical/biological sensing communities due to their capability of sensitivity improvement. It is important to know that a fast, reliable, high-sensitivity and cost effective biosensor would enable us to effectively impede the harmful substances around us, preventing potential disastrous outcomes. Recently, Luther et al. demonstrated that the absorbance obtained from the cation-deficient semiconductors results from LSPR of free holes. They found that in the case of Cu2-xS, the wavelength of the LSPR absorbance peak is very sensitive to the concentration of free holes. The Manna group investigated the possibility of reversibly tuning the absorption band of Cu2-xSe. They could tune the hole-implicated LSPR peak of Cu2-xSe by oxidizing the NCs using a cerium (IV) complex. These novel nanocrystals have also been shown to have potential in biomedical applications. Hessel et al. and Liu et al. have shown the potential of Cu2–xSe as a potent photothermal therapeutic agent, and as a powerful contrast agent for photoacoustic imaging, respectively. However, these heavily doped semiconductor NCs have not yet been used for chemical sensing purposes. In this abstract, detection and quantification of heavy metal ion (e.g.Pb2+) using Cu2-xS NC will be introduced.
Biography:
Munawar Hussain worked as assistant professor in chemistry at Islamia University Bahawalpur, Pakistan from 2001-2008. He received his PhD in 2011 and his first post doctorate 2011-12 from University of Vienna, Austria. He has been working as a principal investigator and senior scientist at Tubingen University, Germany since October 2012. He mainly focuses artificial biomimetic materials for pharmaceutical and clinical applications of QCM-D technique. He focuses coagulation assays, haemostais, thrombosis, platelets aggregation, cancer cells and mesenchymal stem cells. He is famous for professional photography and tourism.
Abstract:
QCM-D (Quartz Crystal Microbalance with Dissipation) has outstanding potential for thrombosis and haemostasis studies in therapeutic directions and decisions.It is also attractive as diagnostic tool for clinical challenges in the prospective of cost-effectiveness, robustness and straightforwardness. An important relevant example is Heparin induced thrombocytopenia (HIT) platelet aggregation diagnostics. For HIT today’s clinics rely on functional assays including HIPA tests (heparin induced platelet aggregation) and serotonin release tests. These tests are tedious, time consuming and costly. HIT assays are part of clinical diagnostic but these need high expertise in this area. Owing to tediousness, time consumption and costs such tests should not be performed in laboratories with limited experiences in this area. QCM-D has outstanding potential for HIT detection of destructive antibodies via using ultrasensitive thin films.
Matic Krivec
Carinthian Tech Research, Austria
Title: Biosensor based on CYP2D6-functionalised carbon nanotube transducer for continuous detection of xenobiotics
Time : 15:20-15:40
Biography:
Matic Krivec, obtained his Ph.D. degree in 2014 at Jozef Stefan International Postgraduate School (Slovenia) in the field of nanosciences and nanotechnologies and is currently a full-time junior researcher at CTR Carinthian Tech Research AG in Austria. His recent research topics are synthesis and characterization of nanoparticles, in particular TiO2 nanoparticles and carbon nanotubes, and their integration into smart devices (microreactors, gas sensors, electrochemical sensors). He is an author and co-author of 5 publications in peer-reviewed journals, 18 publications in conference proceedings and one PCT patent application.
Abstract:
The rising demand of personalised therapeutic products has led to an increasing interest in the investigation of sensing approaches for the detection of drugs, food compounds, and natural substances including potential mutual interferences. Although enzyme-based electro-chemical bio-sensors show significant potential towards the construction of a sensitive and selective sensor, a direct quantitative detection of the enzyme’s catalytic activity still remains challenging. Distinct properties of the enzyme are required as well as an electrode in order to enhance the direct electron transfer (DET) between the enzyme’s catalytic active site and the electrode. Cytochrome P450 enzymes (CYPs) comprise for about 80% of the Phase-I drug-metabolizing enzymes in the human liver, whereas they functionalize chemicals that are foreign to the human body (xenobiotics) and endogenous substrates in order to increase their excretion. In our study, a screen printed working electrode with an additional carbon nanotube layer was functionalised with microsomes prepared from insect cells infected with recombinant baculovirus containing human isoenzyme cytochrome P450 2D6 (CYP2D6) and cytochrome P450 reductase, using a drop-casting technique and the immobilisation was additionally stabilised using two different cross-linkers, i.e. glutaraldehyde and poly(ethyleneglycol) diglycidyl ether, and an additional stabilising agent Nafion. The characterisation of the biosensor was conducted in a micro-cell, the activity of the immobilised CYP2D6 enzyme was measured by conversion of two model substrates, i.e. Luciferin-ME EGE and dextromethorphan, where the concentration of the products, i.e. Luciferin EGE and dextrorphan, were determined using high-performance liquid chromatography. The stability, selectivity and sensitivity of the biosensor were evaluated using a cyclic-voltametrical and chronoamperometrical processing of the induced signal.
Christopher Edozie Sunday
University of Western Cape, South Africa
Title: Application of gold nanoparticles-dotted 4-nitrophenylazo graphene in a label-free impedimetric deoxynivalenol immunosensor
Time : 15:55-16:15
Biography:
Christopher Edozie Sunday is currently a teaching assistance at University of Western Cape, South Africa. His research interests include Analytical Chemistry, Spectroscopy, HPLC, Electrochemistry, FTIR
Abstract:
We report a new concept to construct a label-free electrochemical inhibition-based immunosensor for the detection of the mycotoxin deoxynivalenol (DON) in cereal samples. The electrochemical impedance spectroscopy of tris(bipyridine) ruthenium (II) chloride was used as a marker enhanced with gold nanoparticles-dotted 4-nitrophenylazo functionalized graphene (AuNp/G/PhNO2) nanocatalyst mediated in Nafion on a glassy carbon electrode. Under the optimized conditions, the formation of immunocomplexes inhibited electron flow and increased the charge transfer resistance of the sensing interface linearly. The change in impedance was proportional to DON concentrations in the range of 6–30 ng/mL with a sensitivity and detection limit of 32.14 ΩL/ng and 0.3 μg/mL, respectively, which compares favorably with the ELISA result. The proposed sensor had a stability of 80.3%, good precision and selectivity in DON standard solution containing different interfering agents, indicating promising application prospect for this strategy in designing impedimetric, electrochemiluminescent, voltammetric or amperometric sensors. Biography:
Xiaoyu Ma
University of Connecticut, USA
Title: Fluorescent protein membrane based biosensor for ultrasensitive heme/hemin detection
Time : 16:15-16:30
Biography:
Xiaoyu Ma is a Ph.D. student in the Department of Biomedical Engineering, University of Connecticut, USA. She earned her Bachelor degree in 2012 from Northwest A&F University, China. Her research concentrates on biodegradable hydrogel for biomedical applications.
Abstract:
Malaria plagues seriously in some tropical parts of the world, partly due to thelack of low-cost, sensitive diagnostic tools accessible.A simple way for fast monitoring malaria infection is to detect the elevated level of heme/heminin the blood serum. In this study, fluorescent protein nanoparticles were prepared and then employed to develop a novel optical biosensor for heme/hemin detection.The as-prepared protein nanoparticles were characterized by various advanced technologies. Its application for ultrasensitive heme/hemin detection was also demonstrated as the fluorescence intensity of the protein nanoparticles were quenched significantly upon the titration of hemin. The ultrasensitive sensing performance is ascribed to Photo-induced Electron Transfer (PET) as well as specific interactionbetween hemin and the fluorescent protein nanoparticles. The present study provides insights into the design of a cheap, simple and highly sensitive heme/hemin fluorescence biosensor which holds great potential for a broad spectrum of applications.
Gogalic Selma
Austrian Institute of Technology, Austria
Title: A multiplexed protein based urine chip to distinguish recurrent from non-recurrent BCa
Time : 16:30-16:45
Biography:
Selma Gogalic hascompletedher master of science and engineeringat the age of 23 years. In january 2013 she started her PhD at the Universityof Vienna/AustrianInstitute of Technologyon “multimodularbiomarkeranalysisworkflow for diagnosis, prognosis and monitoring of drug treatment response in bladder cancerâ€. Part of this work hasbeenpublished in peerreviewedjournals. Currentlysheisalsolecturerat the University of AppliedSciencesin St.Pölten (Digital Healthcare) for measuring- and analyticalmethods in healthcaresystems.
Abstract:
Cancer of the bladder (BCa) is killing thousands of people a year. It is the fourth leading cause of cancer in men (US) and the costs for treatment are rising due to high relapse rate (50% within 2 years). To intervene recurrence of BCa routine cytology and cystoscopy are done, representing the gold standard. Nevertheless these diagnostic tools are expensive, time consuming, invasive and lead to urinary infections in up to 16% of patients. Therefore detection in urine to monitor recurrent BCa is in focus. Commercial urine based tests measure single markers, such asNMP-22 and BTA. However, none of them has proofed good sensitivity and specificity. To overcome the problem of low sensitivity and specificity we have developed a protein microarray based on a unique panel of 10 biomarkers. This BCa chip consists of ARChip Epoxyglass-slidesspotted with captureantibodies to bindthe respective biomarkers. The binding is then detected with a secondary biotinylated antibody and Dy647-streptavidin (simultaneouslywithin5hrs). The chip was validated using samples of 76 patients. ROC curves were generated and the optimal cut-off value determined. Expression of fourbiomarkers(DCN, VEGF, IL-8, EN-2) out of 10wassignificantlydifferentbetweenpatientsamples with recurrent and non-recurrent BCa. Those are actually subject to a multi center study with 1013 patients.
Leena Mattsson
Austrian Institute of Technology, Austria
Title: Development of enzyme, antibody and MIP based assays for the rapid detection of biogenic amines
Time : 16:45-17:00
Biography:
Leena Mattsson graduated as a master of science in technology from University of Turku, Finland, in 2013 with the focus of in vitro diagnostics and label technologies. Since February 2014 she is working in Austrian Institute of Technology on her PhD thesis in the field of biosensors and food analytics.
Abstract:
Recent trends in food safety promote the use of biogenic amines as markers for food quality and process hygiene. Biogenic amines are naturally occurring substances especially in fish and fermented food due to bacterial activity, and are mainly produced during storage or processing. Hence there is a need for a rapid, simple and low-cost test system for routine use. We demonstrate various approaches for detection of biogenic amines, starting with histamine: immunoassay on a microarray format, biomimetic recognition and enzymatic detection. Competitive immunoassay on a microarray was established by immobilizing hapten-protein conjugates on a reactive biochip. From various conjugation strategies (carbodiimide, glutaraldehyde cross-linking and coupling to oxidized glycoproteins or using microbial transglutaminase) the carbodiimide chemistry was the most reproducible and stable. The dynamic range of the assay was set to be around the defect action level of histamine (50 µg mL-1). Microarray format will enable multiplexing with other biogenic amines such as putrescine, tyramine and spermine. Diamine-oxidase oxidizes biogenic amines consuming oxygen and producing ammonia and peroxide. The amount of peroxide was measured colorimetrically using HRP with chromogenic substrates. Histamine binding molecularly imprinted polymers (MIPs) were synthesized by precipitation polymerization. The binding occurred in aqueous solution and was detected using spectrophotometric, gravimetric and fluorescence detection. The binding properties and the size of MIPs were controlled with the change of functional monomers (as methacrylic and acrylic acid) and solvent volume. The different approaches were studied in terms of stability and reproducibility, as well as their applicability for rapid food quality monitoring.
Biography:
Bo Liu ( received Master and Bachelor degree at Technische Universität Dresden, Germany), PhD student at Chemnitz University of Technology in Germany.
Abstract:
The development of electronics such as implantable computing, biosensor and medical equipment demands new energy resources which would be utilized in the human body safely and stably with high energy and capacity densities, which is a major challenge for the present battery technology. Glucose, as a natural product, is produced in a huge quantity every year in the plant. In glucose molecule storage high energy, which could be released by enzymes. Glucose biofuel cell (GBFC) produces electricity through catalyzing glucose by enzyme. GBFC is powered by glucose and enzyme. Glucose exists also in animal fluids, such as human blood( Glucose level 6.3mM and 4.5L blood). That means we can get energy from our blood. The enormous challenge of GBFC is to improve the power density and discharge time. Because enzymes for this reactions are in vivo enzyme and they are sensitive to pH, temperature and some ions. It is challenge to keep the activity of enzymes out of the cell, to reach a high catalysis ability. we designed a new GBFC based on bilayer rolled-up nanomembrane electrode, to ensure a high activity of enzyme and to reach a continuous catalysis of enzyme, to solve the problem in the previous research. we expect a higher power density and ultralong continuous discharge time in the research.
Mohammed Marie
University of Arkansas, USA
Title: Zinc oxide nanorods biosensors based on electrochemical reaction for glucose sensing
Biography:
Mohammed Marie was born in Iraq, and he finished high school in a small town called Tuz. He joined Baghdad University and obtained bachelor degree in science from physics department.In early 2007, Mohammed received his master degree in materials science which allowed him to become a faculty member in Tikrit University, physics department. The title of his thesis was “Study the effects of thickness and annealing on structural, optical, and electrical properties of cadmium sulfide thin films. Mohammed taught two courses which were electricity and magnetism and digital electronics and published three scientific papers as a first and second author. His first paper as a first author was about “Study the effect of irradiation by Co2 laser on dielectric constant of led sulfidethin films. In 2011, Mohammed promoted to an instructor, and he got a scholarship from Iraqi government to finish his Ph.D. in the United States of America.Currently, he is a Ph.D. student in Microelectronics-Photonics graduated program at the University of Arkansas, Fayetteville. Zinc oxide nanorodselectrochemical sensor is his current research work at the electrical engineering department. Zinc oxide can be grown by using sol-gel growth method, and it can be characterized by using scanning electron microscopy, spectrophotometer, and Raman shift. Another area to be investigated is the growth of titanium dioxide nanotubes and compares it with zinc oxide nanotubes. Fabricating and characterizing nanostructureselectrochemical sensors allow Mohammed to combine between materials science, nanotechnology, and biological technology. After finishing his Ph.D., he will go back to his home country to serve at one of the universities as a professor. Mohammed spends his leisure time with his family and reading different articles.
Abstract:
Glucose biosensor based on zinc oxide ZnO nanorods was investigated. A sol – gel technique was used in the fabrication process and the working electrode was deposited on glass coated indium tin oxide with an area 0.3*0.3 cm2. Zinc oxide nanorods were well aligned in a hexagonal structure with variety of diameters between 68 – 116.3 nm as it shown the scanning electron microscopy image. The Raman and absorbance spectroscopy were used to characterize the material. The absorbance optical peak was observed ~ 370 nm corresponding with the band gap of ZnO 3.37 eV and the measured was performed in the range from 300 – 1000 nm. The observed peak in Raman spectrum was at 440 cm-1 matching with the lattice vibration of ZnO. Glucose oxidase GOx and nafion membrane were spin coated with a speed 4000 rotation per minute on top of the nanorods to increase the sensitivity and prevent any undesired chemical reaction. The time response of the fabricated biosensor was 3 sec and the cyclic voltammetry curve illustrates the oxidation reduction process of glucose by GOx and the oxidation peak was around 0.5 V. The obtained sensitivity was 10.911 mA/mM cm2 and the lower limit of detection was 0.22 µM which indicates the high performance of the fabricated ZnO biosensor.
Pawan Jolly
University of Bath, United Kingdom
Title: Electrochemical DNA-based sensor arrays for prostate cancer biomarker detection
Biography:
Pawan Jolly is a Marie Curie Research fellow at the University of Bath, UK and in my final year of Ph.D. I completed my postdoctoral studies from Aachen University of Applied Sciences, Juelich, Germany and was involved as a student research associate on project related to field effect biosensors. I did my internship at Philips Research, Eindhoven, the Netherlands on opto-magnetic biosensors. I did my bachelors studies from Amity University, Noida, India and did many small projects in renowned research institutes of India. I have published 3 papers in reputed journals and submitted 3 more for review.
Abstract:
The need for early diagnosis of prostate cancer with a high sensitivity and accuracy has led to increasing work on the detection of multiple biomarkers for PCa.We report on the development of novel techniques to detect different biomarkers for PCa using aptamers. In this study various detection methods are being investigated, namely electrochemical impedance spectroscopy, surface plasmon resonance, Open circuit potential and colorimetric techniques. Novel surface modification strategies are also being developed, e.g. impedimetric detection of prostate specific antigen (PSA) with aptamers together with a thiol-terminated sulfo-betaine, which may allow detection of PSA levels down to 0.5 ng/mL. Additionally, in order to increase the sensitivity of PSA aptasensor, a novel assay is being developed where a PSA-binding antibody is used in conjunction with a DNA aptamer to perform a sandwich assay using the colorimetric technique. We have not only developed for quantification of PSA but have also developed novel assay for glycoprofiling studies. Another novel approach that is being developed is synthesis of synthetic receptors using molecularly imprinted polymers integrated with aptamers. As part of the search for enhanced PCa diagnosis, the detection of the biomarker α-methylacyl-CoA racemase (AMACR) is also being investigated using DNA aptamers. AMACR levels are consistently increased in early PCa. The best aptamer sequence for an on-surface detection of the AMACR protein has been identified using surface plasmon resonance (SPR). We have also developed novel detection method for Micro RNAs (miRNAs) by exploiting the inherent charges of nucleic acids and an amplification method with positively charged gold nanoparticles.
Sanghamitra Mandal
University of Arkanas, USA
Title: GOx detection based on amperometric response of electrochemical glucose biosensor using hydrothermal sol-gel synthesized ZnO nanorods
Biography:
Sanghamitra is pursuing herdoctoral degree in Electrical Engineeringfrom the University of Arkansas, Fayetteville, USA. Her area of expertise includes semiconductor device physics. Current research interest is focused on synthesis of nanomaterials for the fabrication of electrochemical sensors. She is trained on using optoelectronic devices like the scanning electron microscope, spectrophotometer, optical surface profiler, and Ramanmicro spectrometer.In 2013, Sanghamitra completed her Master degree in Semiconductor Photonics Engineering from the University of Sheffield, England. She graduated with her Bachelor degree
Abstract:
This paper describes the fabrication of a glucose sensing biosensor.The working principle is based on the electrochemical reaction taking place between immobilized glucose oxidase adsorbed by the ZnO nanorods, and the electrolyte glucose.Zinc oxide (ZnO) nanorods were synthesized on indium titanium oxide coated glass substrate using the hydrothermal sol-gel growth technique. Characterization of the ZnO nanorods is performed using the absorption spectroscopy, micro-Raman spectroscopy and scanning electron microscopy. A blue shift is observed in the optical band gap due to increased particle size and density of the ZnO nanorods compared to bulk ZnO.The two-electrode system is employed to measure current for sensing the glucose concentration inside an electrochemical cell. Nafion/GOx/ZnO nanorods/ITO is used as the working electrode, and platinum plate as the reference electrode. Amperometric response for clinical range of blood glucose concentration from 0.25 ï€ï€ 20 mM is measured at 0.8 V. The response time for the proposed biosensor is identified to be less than 3 s. For a linear range of glucose concentration from 0.25ï€ ï€ 7.5mMthe analyzed sensitivity is 51.3 µA/cm2 mM-1,and the lower detectionlimit is5 µM. The electrochemical characterization of the biosensor is performed using the cyclic voltammetry method for a voltage range from -0.2 ï€1 V at a scan rate of 0.1 V/s. The achieved results indicate that ZnO nanorods based working electrode demonstrate much higher current response, excellent stability, reusability, and faster response time than previously reported enzymatic electrochemical biosensors.
Shengyuan Deng
Nanjing University of Science & Technology, China
Title: Detection of zinc finger protein (EGR1) based on cathodic electrochemiluminescence of nanoclay-supported porphyrin
Biography:
Shengyuan Deng completed his PhD from Nanjing University. He is currently an Assistant Professor at NUST. His research involves molecular diagnosis, biomedical sensing, biomaterials, elecctroluminscent and optoelectronic (bio)transducers. He has published more than 25 papers in reputed journals.
Abstract:
Early growth response protein 1 (EGR1), as a characteristic example of zinc finger proteins, acts as a transcription factor in eukaryotic cells and is involved in mediating protein-protein interactions. Here, a novel electrochemiluminescence (ECL)-based protocol for EGR1 assay was developed with a new eco-friendly emitter: Nanoclay-supported zinc proto-porphyrin IX (ZnPPIX). It could stimulate an intense monochromic ECL irradiation at 644 nm in the aqueous solution with the dissolved oxygen as an endogenous coreactant. This ECL derivation was rationalized via hyphenated spectroscopy and theoretical calculation. To promote water solubility and solid-state immobilization of porphyrins, the lamellar artificial laponite was employed as a nanocarrier owning to its large specific area without black-body effect. The facile exfoliation of laponite produced quality monolayered nanosheets and facilitated the intercalation of ZnPPIX within, resulting in a highly efficient ECL emission. Based on the release of Zn2+ in zinc finger domains of EGR1 upon contact with the ECL-inactive PPIX, which was monitored by circular dichroism and UV absorption, a sensitive Zn2+-selective electrode for the ″signal-on″ detection of EGR1 was prepared with a detection limit down to 5.8 pg mL−1 and a linearity over 6 orders of magnitude. The proposed porphyrin-based ECL system thus infused fresh blood into the traditional family of ECL nanoemitters, showing great promise in bioassays of structural Zn(II) proteins and zinc finger-binding nucleotides.
Elisabete Fernandes
International Iberian Nanotechnology Laboratory, Portugal
Title: A magnetoresistive biochip platform and its bioanalytical applications
Biography:
Elisabete Fernandes holds a PhD degrees in Chemical and Biological Engineering from the University of Minho, Portugal (2013). Her PhD research focused on the detection of Salmonella using a Phage-based Biosensor, which was developed in collaboration with the University of Auburn (USA), the Katholieke Universiteit Leuven (Belgium) and the International Iberian Nanotechnology Laboratory (INL). In 2013 she obtained a Marie Curie Fellowship under the European project: PROSENSE “Cancer Diagnosis: Parallel Sensing of Prostate Cancer Biomarkersâ€. As a post-doc researcher, she worked at École Polytechnique Fédérale de Lausanne (EPFL) in the development of a Microfluidic device for blood plasma separation, and at Xeptagen S.p.A in the development of a pre-commercial portable medical analysis system for early cancer detection. She has publications in scientific journals, an international patent and several communications in proceedings of international conferences. Her ambitious goal is to obtain a solid knowledge in the area of biosensors, and apply in the current/urgent challenges.
Abstract:
Cancer, stroke and cardiovascular diseases are excellent examples of large public health problems requiring an early detection system that led scientific communities focus their interests on the biosensing area. Integrated spintronic biochip platforms are being developed for pointofcare (POC) diagnostic/prognostic applications. Here we report a biochip platform comprising 30 spin-valve (SV) sensors separated in six groups of 4 bioactive sensors plus a reference sensor. This platform join all the advantages of a magnetoresistive based biochip sensitivity, fast response, no signal interference with the possibility to perform an integrated sample pretreatment (separation, labelling and amplification) in a miniaturized, portable, lab on a chip electronically assisted platform. This work presents the potential of this technology on different bioanalytical applications reported in several publications (e.g. proteins, pathogens, DNA identification and polymorphism), emphasizing a multiplex detection system that has being performed to detect a panel of biomarkers present in human serum to predict brain ischemia. Our detection strategy is based on multiple “sandwich†immunoassays over the chip area. The biological targets are initially labelled in serum by using antibodyanchored magnetic nanoparticles (MNPs) of 250nm and loaded through microfluidics onto an array of SV sensors, where the specific probes (antibodies) are immobilized. Upon probe/target recognition, and a washing step to remove unspecifically bound biomolecules, the final immobilized target is quantified. The full assay takes around 45 minutes. Calibration curves are being established to define the detection limits for each of the six stroke’s biomarkers, and we have reached the minimum detection limit of 4 μg/mL for Fibronectin, which is in accordance with the cutoff value defined for stroke patients. Taking the advantages of the magnetic attraction that allows to concentrate the target over the probe sites, limits of detection in the nanogram range are further expected.