Day 1 :
Keynote Forum
C Donald Combs
Eastern Virginia Medical School, USA
Keynote: Data analytics, the digital patient and simulation in healthcare
Time : 10:30 - 11:00
Biography:
C Donald Combs, PhD serves as Vice President and Dean, School of Health Professions, at the Eastern Virginia Medical School (EVMS). He holds faculty appointments as tenured Professor of Health Professions at EVMS, Professor of General Medicine at the State Medical and Pharmaceutical University “Nicolae Testemitsanu”, Visiting Professor of Medical Simulation at University of Paris—Descartes and as Adjunct Professor of Modeling, Simulation and Visualization Engineering at Old Dominion University. From 1996 to 2002, he also served as a Senior Fellow at the US Naval Postgraduate School.
Abstract:
The dramatic growth in data about the human body and the human in social context combined with the progress in informatics, and modeling and simulation present an opportunity to realize a thirty-year old vision for a virtual human. This virtual human, however, will be far more sophisticated than the initial vision in that it will be capable of serving as a platform for research, education, patient care, drug and device testing. It will also be capable of accurately representing individuals and populations over time for purposes of screening, prevention, treatment, and analysis. The more accurate descriptor is to think in terms of a digital patient platform with infinite combinatorial possibilities. The objectives of this presentation are to provide an update on the status of a variety of international research efforts to extend human physiome research to include the social, behavioral and societal systems necessary to construct the digital patient and to discuss the opportunities to developing new models and simulations that will use the digital patient platform. The goal of the digital patient is to develop a database, software and analytic framework that integrates biological systems (body, organ, tissue, cells, molecules), a variety of scientific disciplines (biology, physiology, biophysics, biochemistry, molecular biology, bioengineering or social science), anatomical sub-system (cardiovascular, musculoskeletal, gastrointestinal, etc.), social context and data analytics. This presentation provides an overview of the major research initiatives that are underway, and the challenges that must be addressed, particularly in the use of biosensors and the aggregation of data into usable formats.
Keynote Forum
Mark Bradley
University of Edinburgh, UK
Keynote: Optimal reporters for electrochemical detection of protease activity
Time : 11:00 - 11:30
Biography:
Mark Bradley was made a Professor of Combinatorial Chemistry in 1996. He has been elected to fellowships of both the Royal Society of Chemistry and Edinburgh and has awarded a number of prizes such as the Novartis Chemistry Lectureship, the Award from the Society of Combinatorial Sciences, the 2015 Royal Society of Chemistry Tilden Prize. His group has published over 300 peer reviewed papers and more than 60 PhD students have graduated from his group. He holds an ERC Advanced grant and is PI on £18M of active grants. He has published over 300 peer reviewed papers, filed some 20 patents and is co-founder of Ilika Technologies (IPO on AIMS May 2010) and of Edinburgh Molecular Imaging (2014). rn
Abstract:
Electrochemical, peptide-based, biosensors are attracting significant attention for the detection and analysis of proteins. Among them, proteases are interesting targets due to their relevance in many pathophysiological conditions, including inflammation and cancer. Different platforms have been suggested for the electrochemical detection of these enzymes, as they catalyze the cleavage of amide bonds at specific sites in a protein or peptide. To meet the increasing demand for selective and sensitive analytical tools for the detection of proteases, improved analytical features, such as reduced limits of detection and sensor stability, are important. Here we investigate the effect of the nature and length of a spacer on the sensitivity of the electrochemical sensor. Redox-tagged peptides, tethered to a gold surface through a self-assembled monolayer (SAM), were selected as the Biosensing platform for the electrochemical detection of protease activity.
Keynote Forum
Andreas Hütten
Bielefeld University, Germany
Keynote: Magnetic nanoparticles meet microfluidics
Time : 11:45 - 12:15
Biography:
Andreas Hütten has completed his PhD in Physics from Göttingen University in Germany and was working as a Feodor Lynen Research Fellow at UC Berkeley and Lawrence Berkeley National Laboratory in the US and in Germany at the Leibnitz Institute for Solid Sate and Materials Research Dresden. He is Professor of Thin Films and Physics of nh at the Department of Physics at Bielefeld University in Germany. He has published more than 190 papers in reputed journals and is serving as an Editorial Board Member for sensors.
Abstract:
Lab-on-a-chip immunoassays utilizing super paramagnetic beads as labels allow for transport, separation and detection of different bead species by employing magnetic strayfield landscapes and/or special channel geometries in microfluidic systems in combination with integrated magnetoresistive sensors. Moreover, the formation of magnetic bead superstructures due to dipolar magnetic interactions can be applied as configurable matter so as to realize programmable microfluidic functions such as mixers, filters or valves which are able to simultaneously detect biomolecule via molecular recognition. This lecture is focused on physical aspects regarding microfluidics and the formation of magnetic bead superstructures which play an important role on the way to magnetically controlled lab-on-a-chip structures. Moreover, the realization of giant magnetoresistive Biosensors based on these magnetic bead super structures will be discussed in detail.
- Biochips & Nucleic Acid Sensors
Types of Biosensors
Chair
Zuzana Bilkova
University of Pardubice, Czech Republic
Co-Chair
Valery Pavlov
Centro de Investigación Cooperativa en Biomateriales CIC biomaGUNE, Spain
Session Introduction
C Donald Combs
Eastern Virginia Medical School, USA
Title: Data analytics, the digital patient and simulation in healthcare
Time : 10:30 - 11:00
Biography:
C Donald Combs, PhD serves as Vice President and Dean, School of Health Professions, at the Eastern Virginia Medical School (EVMS). He holds faculty appointments as tenured Professor of Health Professions at EVMS, Professor of General Medicine at the State Medical and Pharmaceutical University “Nicolae Testemitsanuâ€, Visiting Professor of Medical Simulation at University of Paris—Descartes and as Adjunct Professor of Modeling, Simulation and Visualization Engineering at Old Dominion University. From 1996 to 2002, he also served as a Senior Fellow at the US Naval Postgraduate School.
Abstract:
The dramatic growth in data about the human body and the human in social context combined with the progress in informatics, and modeling and simulation present an opportunity to realize a thirty-year old vision for a virtual human. This virtual human, however, will be far more sophisticated than the initial vision in that it will be capable of serving as a platform for research, education, patient care, drug and device testing. It will also be capable of accurately representing individuals and populations over time for purposes of screening, prevention, treatment, and analysis. The more accurate descriptor is to think in terms of a digital patient platform with infinite combinatorial possibilities. The objectives of this presentation are to provide an update on the status of a variety of international research efforts to extend human physiome research to include the social, behavioral and societal systems necessary to construct the digital patient and to discuss the opportunities to developing new models and simulations that will use the digital patient platform. The goal of the digital patient is to develop a database, software and analytic framework that integrates biological systems (body, organ, tissue, cells, molecules), a variety of scientific disciplines (biology, physiology, biophysics, biochemistry, molecular biology, bioengineering or social science), anatomical sub-system (cardiovascular, musculoskeletal, gastrointestinal, etc.), social context and data analytics. This presentation provides an overview of the major research initiatives that are underway, and the challenges that must be addressed, particularly in the use of sensors and the aggregation of data into usable formats.
Andreas Hütten
Bielefeld University, Germany
Title: Magnetic nanoparticles meet microfluidics
Time : 11:45 - 12:!5
Biography:
Andreas Hütten has completed his PhD in physics from Göttingen University in Germany and was working as a Feodor Lynen Research Fellow at UC Berkeley and Lawrence Berkeley National Laboratory in the US and in Germany at the Leibnitz Institute for Solid Sate and Materials Research Dresden. He is Professor of Thin Films and Physics of Nanostructures at the Department of Physics at Bielefeld University in Germany. He has published more than 190 papers in reputed journals and is serving as an Editorial Board Member for sensors
Abstract:
Lab-on-a-chip immunoassays utilizing super paramagnetic beads as labels allow for transport, separation and detection of different bead species by employing magnetic strayfield landscapes and/or special channel geometries in microfluidic systems in combination with integrated magnetoresistive sensors. Moreover, the formation of magnetic bead superstructures due to dipolar magnetic interactions can be applied as configurable matter so as to realize programmable microfluidic functions such as mixers, filters or valves which are able to simultaneously detect biomolecule via molecular recognition. This lecture is focused on physical aspects regarding microfluidics and the formation of magnetic bead superstructures which play an important role on the way to magnetically controlled lab-on-a-chip structures. Moreover, the realization of giant magnetoresistive sensors based on these magnetic bead super structures will be discussed in detail.
Zuzana Bilkova
University of Pardubice, Czech Republic
Title: Alkaline phosphatase or Q dots labeled antibody-based electrochemical biosensors for ultrasensitive tumor markers detection
Time : 12:20 - 12:40
Biography:
Zuzana Bilkova graduated in Molecular Biology and Genetics from Charles University, Prague (CZ). In 2000, she received PhD in Analytical Chemistry - Immunochemistry and in 2005 she became Head of Department of Biological and Biochemical Sciences at University of Pardubice. In 2014, she was promoted to Professor of Clinical Immunology. Her scientific expertise: Immunochemistry, bioaffinity chromatography, structural analysis of clinically relevant biomarkers using modern (nano)materials as a solid phase combined with microfluidic systems and electrochemical detectors. Till date, she has published more than 80 papers in scientific journals and she gave more than 30 invited lectures at conferences.
Abstract:
Even though there are a lot of options in treating gynaecological malignancies, ovarian cancer still remains a leading cause of death. The lack of precise early warning signs is one of the factors that further contribute to the fact that only 25% of ovarian tumors are identified at stage I. Thus diagnosis at an early stage is the most important determinant of survival. Currently, serum CA125 is the most commonly used tumor marker for ovarian cancer, however the sensitivity of CA125 is limited. Next promising tumor marker for ovarian cancer is HE4, low molecular-weight glycoprotein, expressed primarily in epithelia of normal female genital tissues and overexpressed in epithelial ovarian cancer. Diagnostic tools applied today at clinics have had very limited success in early detection of these markers. Nowadays, there is the push to combine the highly specific immunochemical methods with highly sensitive electrochemical Biosensors. Selectivity and proper specificity provided by polyclonal or monoclonal antibodies in combination with electrochemical detection bring excellent analytical features. Recent attention has focused on advanced IgG labelling enabling the combination with electrochemical biosensing. The enzyme alkaline phosphatase or semiconductor nanocrystals Qdots are some of examples how to obtain desired sensitivity using square wave voltammetry resp. stripping voltammetry techniques. These advanced methodologies enable to detect and quantify the tumor markers in femtoMole required sensitivity.
Sheila Sadeghi
University of Torino, Italy
Title: An electrochemical biochip based on human hepatic drug metabolising enzymes in the presence of graphene and/or AuNps
Time : 12:40 - 13:00
Biography:
Sheila Sadeghi was awarded her PhD from Imperial College London, UK, studying the electrochemistry of haem proteins followed by Post-doctoral studies at Imperial College and University of California at San Francisco. She was awarded The JBC/Herb Tabor Award in 2014 for her work on the development of a P450-based bioelectrochemical platform for investigation of drug-drug interactions of human hepatic CYP3A4. She is currently an Associate Professor of Biotechnology in University of Torino. She has published more than 50 papers in reputed journals and has been serving as an Editorial Board Member of the Journal of Biotechnology and Applied Biochemistry since 2011.
Abstract:
Human hepatic Phase I drug metabolising enzymes include the large family of cytochromes P450 and flavin-containing monooxygenases (FMO). In general, the activity of these enzymes renders the drugs and xenobiotics more amenable to excretion. Cytochromes P450 and to a lesser extent FMOs are important in the drug discovery process carried out by pharmaceutical companies worldwide since more than 80% of currently available drugs are substrates and/or inhibitors of these enzymes and any new chemical entity has to be tested against these monooxygenases in order to determine their clearance as well as toxicity. Both these family of monooxygenases are also redox proteins with either haem or flavin as their cofactors. The presence of the latter makes these enzymes responsive to electrochemical methodologies. However, these enzymes are membrane-bound and difficult to manipulate. Our group has been involved in studying the wiring of these human enzymes to electrode surfaces for the last two decades. Electrode modification for immobilisation of these enzymes has been achieved in a number of ways, ranging from the use of films of positively charged surfactants such as didodecyl ammonium bromide (DDAB), poly-(dimethyldiallyl ammonium chloride) (PDDA) to ordered self-assembled thiol-terminated chains such as cystamine-maleimide. More recently, we have also demonstrated the successful use of gold nanoparticles (AuNps) and graphene. Data will be presented regarding the activity as well as identification of the metabolite(s) produced by these human enzymes and their polymorphic variants once immobilised on electrode surfaces in the presence of graphene. Finally, development of this type of biochips has direct biosensor applications in the era of personalized medicine.
Ritu Sharma
Malaviya National Institute of Technology, India
Title: A review on surface plasmon resonance and its application as biosensing element
Time : 13:00 - 13:20
Biography:
Ritu Sharma has completed his PhD in the year 2011 from Malaviya Institute of Technology, Jaipur, India. She is working as Assistant Professor in Department of Electronics and Communication, MNIT Jaipur. She has work experience of 17 years published more than 35 papers in various reputed journals and international conferences and has been serving as a reviewer of many journals of repute.
Abstract:
Surface plasmon resonance (SPR) is an optical phenomenon which takes place at dielectric and metal interface. The light at a particular angle is incident on the metal surface and when the frequency of light coincides with the vibration frequency of electrons, resonance occurs. This resonance is confined at the interface. In this review paper various aspects of surface plasmon, their generation, and their material dependency is reviewed. The optical generation of surface plasmon due to prism coupling, grating coupling and waveguide coupling is discussed and compared using available literature. Then SPR application as biosensing element is reported. SPR-based biosensors generally deal with the study of biomolecular interactions, detection of cancer biomarkers and other human diseases and also screening of inhibitor of tumor targets. A biosensing element is designed using different metal coating of gold, silver and aluminium of thickness 50nm. The simulation of the biosensing element design has been carried out to show the effect of addition of protein on the reflectance versus incident angle on the three materials coating i.e. Gold, Silver and Aluminium. The results shows that a maximum shift in resonance angle (Δθres = 6.500) occur for gold coating and therefore gives better sensitivity than other material coating.
May C Morris
Institut des Biomolecules Max Mousseron, France
Title: Fluorescent carbon nanotube biosensors for probing intracellular kinase hyperactivation in human cancer
Biography:
May C Morris obtained her PhD in Biology and Health Sciences at the University of Montpellier in 1997 and completed her Post-doctoral training at the Scripps Research Institute, La Jolla, USA. In 2000, she was hired by the CNRS and returned to the Centre of Research on Macromolecular Biochemistry in Montpellier, France. In 2005, she established her own research group and in 2010, she was promoted as CNRS Research Director. In 2014, she moved to the Institute of Biomolecules Max Mousseron, where she is currently in charge of the “Biosensors and Inhibitors” group within the Department of Amino Acids, Heterocycles, Peptides and Proteins for Health. She was awarded the CNRS Bronze Medal in 2006 and the “Scientist of the Future” award from Languedoc-Roussillon Region in 2009. She has published over 60 articles in peer-reviewed journals, edited a volume on “Fluorescent Biosensors” (Elsevier Press) in 2013 and a special issue on “Fluorescent Biosensors in Biotechnology” Journal in 2014. She is currently an Editorial Board Member of ChemBioChem and Frontiers in Chemistry.
Abstract:
Cyclin-dependent kinases (CDK/Cyclins) play a central role in coordinating cell growth and division and are frequently deregulated in cancer, thereby constituting proliferation biomarkers and attractive pharmacological targets. However, probing and quantifying the hyperactivity of these kinases remains challenging, and there are no technologies available to monitor their activity in living cells in a non-invasive fashion. To this aim, we have developed a family of fluorescent biosensors, known as CDKACT, through conjugation of environmentally-sensitive probes to synthetic peptides which are specifically recognized by CDK/Cyclins and undergo fluorescent enhancement upon phosphorylation. We have further conjugated these peptide biosensors at the surface of multiwall carbon nanotubes to obtain self-cell-penetrating sensors of intracellular kinase activity. We show that these carbon nanotube peptide conjugates report on CDK/Cyclin activities in a sensitive and robust fashion in vitro. Moreover, these nanobiosensors penetrate readily into living cells and enable detection and quantification of the intracellular activities of these kinases by fluorescence imaging. This new generation of hybrid carbon nanotube peptide biosensors constitute attractive tools for cancer diagnostics and for evaluating response to therapeutics. They are particularly well suited for molecular imaging and are currently being implementing to monitor CDK/Cyclin hyperactivity associated with cancer progression and inhibition in mouse cancer models
- Biosensors
Types of Biosensors
Bioinstrumentation & Equipments
BioMEMS/NEMS
Nanotechnology in Biosensors
Transducers in Biosensors
Chair
Subrayal M Reddy
University of Central Lancashire, UK
Co-Chair
Valery Pavlov
Centro de Investigación Cooperativa en Biomateriales CIC biomaGUNE, Spain
Session Introduction
Dario Mager
Karlsruhe Institute of Technology, Germany
Title: eDisc – Getting 21st century technology into lab on disc applications
Time : 14:05 - 14:25
Biography:
Dario Mager has studied Microsystem technology (MEMS) at the University of Freiburg. He also obtained his PhD there in the field functional inkjet printing for the direct manufacturing of microstructures. Currently, he is a group leader at the Karlsruhe Institute of Technology in the field of low-cost MEMS where he is focusing on the modification of existing technology to suit new biosensors applications. Mainly using technology adapted system designs for functional inkjet printing and embedded systems.
Abstract:
Lab on a Disc (LoaD) analysis has seen an incredible increase in possible applications and an improvement in quality over the last decade. It allows for testing of a vast variety of features. It has become so successful and reliable that global players have developed products based on LoaD technology for the market. Nearly all these devices, both lab equipment as well as commercial products have in common, that they are based on a purely passive rotating disc and stationary biosensors and actuator components. This approach has three major disadvantages: 1. it requires highly sensitive and powerful sensors and actuators, placed at a distance from the disk and while the disc is spinning these components only have access to the region of interest for short periods, 2) there is no real continuous online monitoring and hence also not a real feedback control of the disc and predefined recipes need to be performed, and 3) Primarily analog electromagnetic signals can be transferred on and off the disc. This is comparable to central heating in houses, where a central oven is switched on and off, and the room temperature is set by setting valves to experience-based positions. House automation based on embedded systems and smart actuators is currently changing this situation at a drastic rate, converting the house from a passive shelter into a highly interactive environment. Based on similar principles, LoaD can profit from the multitude of MEMS sensors and actuators as well as on the computational power available in embedded systems today.
Subrayal M Reddy
University of Central Lancashire, UK
Title: Smart materials:Advances in protein-based molecularly imprinted polymer biosensing
Time : 14:25 - 14:45
Biography:
Subrayal M Reddy completed his PhD from University of Manchester and Postdoctoral studies from University of Wales. He was Lecturer and then Senior Lecturer at the University of Surrey (1998-2015) and has recently taken up an academic position at the University of Central Lancashire (2016). He has published more than 50 papers in reputed peer-reviewed journals and has given numerous invited international conference talks.
Abstract:
Hydrogel-based molecularly imprinted polymers (HydroMIPs) were prepared for several proteins using a family of acrylamide-based monomers. We report HydroMIP binding affinities, in terms of equilibrium dissociation constants (Kd) within the micro-molar range (25±4 µM, 44±3 µM and 17±2 µM for haemoglobin, myoglobin and catalase respectively within a polyacrylamide-based MIP). We have used atomic force spectroscopy to characterize molecular interactions in the MIP cavities using protein-modified AFM tips. Our force data suggest that we have produced selective cavities for the template protein in the MIPs and we have been able to quantify the extent of non-specific protein binding on, for example, a NIP control surface. For the first time, we have also coded the MIPs with a covalently-bound redox tag in order to elicit a direct electrochemical signal in the event of selective protein binding. GC and SPE probes were used for signal transducers and imprinting determination. Co(II)-complex-based MIPs exhibited 92±1% specific binding with protein binding capacities of 5.7±0.45 mg BSA/g polymer and imprinting factors (IF) of 14.8±1.9 (MIP/ non-imprinted (NIP) control). The selectivity of our Co(II)-coded BSA MIPs were also tested using bovine haemoglobin (BHb), lysozyme (Lyz), and trypsin (Tryp). In summary, MIP technologies could provide an inexpensive, fast, and efficient diagnostic biosensor platform highly sensitive, in-situ analysis of biologicals for both environmental and biomedical applications. The author acknowledges UKIERI (IND/CONT/R/12-13/779), the British Council and DST (India) and NERC and RSC_ACTF (NE/J01/7671) for funding this project.
Valery Pavlov
Centro de Investigación Cooperativa en Biomateriales CIC biomaGUNE, Spain
Title: Enzymatic synthesis and etching in situ of gold and semiconductor nanoparticles in biosensing
Time : 14:45 - 15:05
Biography:
Valeri Pavlov obtained a PhD degree in Chemical Engineering in January 2005 from the University Rovira i Virgili, Spain. He worked in the Hebrew University of Jerusalem, Israel, in the group of Professor Itamar Willner as a Post-doctoral researcher. Since October 2006 he continued his Post-doctoral study at the Chemistry Department of University of Heidelberg, Germany. In February 2007, he joined the new research center CIC Bioma- GUNE in San Sebastian as a group leader. His research interests include enzymatic generation of metal and semiconductor nanoparticles, production of new recombinant mutated enzymes and optical bioanalytical assays.
Abstract:
Fluorogenic enzymatic assays are based on the biocatalytic cleavage of bonds between presynthesized organic fluorescent molecules or fluorescent semiconductor nanoparticles, so called quantum dots (QDs) and quenching moieties. They suffer from insufficient quenching of fluorophores by quenchers resulting in high background signals. We pioneered assays relying on biocatalytic formation of CdS QDs with low background signals, higher quantum yield, reduced photo-bleaching and lower costs. The diameter (2-3nm) of the resulting SNPs is controlled by the nature of capping agents. We discovered a facile, mild and inexpensive enzymatic etching method for resizing of CdS QDs. It was found out that the biocatalytic process involving bromide, Horseradish Peroxidase (HRP) and H2O2 decreased the size of semiconductor CdS QDs. Thus, this phenomenon can be applied to resizing of semiconductor CdS QDs under mild physiological conditions and rapid and sensitive detection of H2O2 and HRP It was proven that CdS QDs immobilized on polyvinyl chloride microspheres can be etched biocatalytically too. We discovered for the first time that HRP is able to produce free radicals which oxidize gold nanorods (AuNRs) and semiconductor QDs. We introduced novel bio-analytical assays based on enzymatic etching of inorganic nanoparticles. HRP is able to induce a gradual oxidation of the AuNRs in the presence of trace concentrations of H2O2 and halide ions. As a consequence, other enzymatic reactions, carried out by Glucose Oxidase (GOx) can be easily coupled to the HRP activity assay, thereby allowing for the detection of different amounts of glucose.
Rodica Elena Ionescu
University of Technology of Troyes, France
Title: Acoustic and plasmonic biosensors for the detection of different classes of (bio)molecules
Time : 15:05 - 15:25
Biography:
Rodica Elena Ionescu earned one PhD from the Ben-Gurion University of the Negev, Israel (2004) and a second PhD from the University of Bucharest, Romania (2007). Since 2009, she is an Associate Professor at UTT, France. Between, April 2014 to March 2015, she was invited to manage and conduct electrochemistry research in the frame of a POC 002-026-NRF 2013 project at the Nanyang Technological University, Singapore. Her research activities include biosensors, functionalization of surfaces, synthesis of nanoparticles and their toxicity to living microorganisms. She is co-author of 7 patents, over 40 peer-reviewed manuscripts and 5 book chapters.
Abstract:
Biosensors have attracted a considerable interest since the last decade. The ongoing research effort consists of developing analytical systems for multi-detection purpose, in order to reduce the time of analysis and the volume of required samples. Such goal can be achieved with the help of multiplexed detection of biomolecules. The presentation will discuss the fabrication of micro-structures on glass substrate which can be used for acoustic biosensors and the preparation of gold nanoparticles for high efficient optical detection based biosensors. The acoustic biosensor is based on Quartz Crystals Microbalance (QCM) for which the upper electrode of the quartz has been micro-structured by gold evaporation through a commercial TEM grid. Thus, several structures can be independently bio-functionalized and further used for the sequential detection of different analytes (eg. atrazine and carbofuran) by monitoring the decay of frequency of the whole quartz structure. The optical biosensor is based on Localized Surface Plasmon Resonance (LSPR) detection. This mode of detection is generated by light when it interacts with conductive nanoparticles that are smaller than the incident wavelength. Herein are presented several easy-to-prepare protocols for tuning LSPR wavelength of NPs over the ultraviolet to near-IR region. Well-organized nanoparticles are created by high-annealing temperatures over glass substrates covered by evaporation of mono- or bi-metallic thin layers. Each NPs protocol is investigated for its LSPR performances in sensitive detection of biomolecules (proteins, DNA, pesticides, etc.).
Vladimir Moshkin
Sib-STRIM" LLC Russia
Title: New ultra-sensitive measurement method in ampere- and voltammetry
Time : 15:25 - 15:45
Biography:
Vladimir Moshkin graduated from the Tomsk Polytechnic University (1977) and Post-graduate studies (1990) with a degree in Electrical Measurements. He participated in the development of popular Russian electrochemical analyzers TA-2 and TA-7 (“Technoanalyt” LLC, Tomsk, Russia). Now, he is the Director of "Sib-STRIM" LLC. He has several Russian publications on electrochemical measurements of signals and is the author of several inventions.
Abstract:
A new method of forming an electrochemical signal can significantly increase measurement sensitivity at low hardware cost. A unique feature of the method is the use of capacitive current as an information component, while in the classical pulsed methods capacitive current is interference. The method is based on the inclusion of high-speed current switch circuit which provides a predetermined period and duration of current interrupt. Main properties thus obtained signal is founded. It is shown, that for a large ratio of time the switch was open to a time closed state theoretical gain of sensitivity is equal to this ratio. Practically achievable values of opening and closing times allow to reach the increasing of sensitivity up to several hundreds. The resulting pulse signal (switching current) has good noise immunity, it can be used even in unsteady conditions such as vibration of the working electrode. The conditions of linearity of the switching current vs. the concentration of the element is determined. It is shown that the proposed method organically allows along with the current measurement to measure both resistance and capacitance of the double layer of electrochemical cell. The authors use the proposed method for several years to determine the number of elements: Fe(III), Cu(II), Cr(III), Cr(VI), Ir(IV), etc., both in chronoamperometry and in voltammetry modes. Practical achieved sensitivity for Fe(III) is not above 1 nM / dm3 (50 ng / dm3) using the general-purpose base of components. It is noted that the use of this approach for cells with ultramicroelectrodes is a perspective.
Lingwen Zeng
Wuhan Academy of Agricultural Science and Technology, China
Title: Nucleic acid biosensors for the detection of heavy metal ions
Time : 16:00 - 16:20
Biography:
Lingwen Zeng has received his PhD degree in Genetics from McMaster University, Canada in 1993. From 1994 to 1997, he had his Postdoctoral training in the University of Chicago, USA. He has then worked in three publicly traded companies (Quest Diagnostics Inc, Scios Inc and Genetics Computer Group Inc) in USA as a Research Scientist and Project Manager. He has returned to China in 2001 and joined Institute of Environmental and Food Safety, Wuhan Academy of Agricultural Science and Technology in 2015 as a Principle Investigator and Director General. His research focuses on exploring novel technologies in the areas of diagnosis for human diseases, food and environmental safety and stem cell research.
Abstract:
Heavy metal ions (such as Hg2+, Pb2+, Cu2+, etc.) are widespread pollutants with distinct toxicological profiles that can cause deleterious effects on human health and the environment even at low concentrations. Thus, routine detection of trace amounts of heavy metal ions with high sensitivity is central for environmental monitoring. Conventional methods including cold vapor atomic absorption spectroscopy (CV-AAS), cold vapor atomic fluorescence spectroscopy (CV-AFS) and inductively coupled plasma mass spectroscopy (ICP-MS) have been widely used for heavy metal ion detection. Although they offer high sensitivity and accuracy, those analytical techniques require expensive and sophisticated instrumentation, skilled personnel and time-consuming sample pretreatment processes, which limit their wide applications in routine measurements. Thus, it is highly desirable to develop on-site biosensors for heavy metal ion detection without instrumentation. In our lab, we focus on developing nucleic acid biosensors for heavy metal ion detection using DNAyzmes, aptamers, and G-quadruplex as the molecular recognition elements. Employing colorimetric analysis, lateral flow strip biosensor, glucometer readout, and fluorescence detector as the sensing platform, our proposed biosensors would be promising strategies for heavy metal ion detection which offer prominent advantages of improved sensitivity, convenience and no need for the washing and separation steps during on-site applications.
Ji-Yen Cheng
Research Center for Applied Sciences, Taiwan
Title: LumiSense - A portable water pollutant monitoring system using whole cell array
Time : 16:20 - 16:40
Biography:
Ji-Yen Cheng received his PhD degree in Chemistry Department of National Taiwan University. After graduation in 1998, he then started his Post-doc research on the DNA microarray in Institute of Biomedical Sciences in Academia Sinica Taiwan. In 2001 he became an Assistant Researcher in Research Center for Applied Sciences in Academia Sinica and was promoted to research fellow in 2013. His research interest is in the biological applications of microfluidics. Some specific topics include the following: Cell response in weak DC EF (electrotaxis)/ Rapid prototyping of microfluidic biochip using laser micromachining/ nanostructure biosensor/ and portable whole-cell sensor.
Abstract:
Whole cell sensors have been proposed as a powerful tool to detect class-specific toxicants based upon their biological activity and bioavailability. A whole cell sensor uses live cells as the sensing elements for harmful chemicals. In this talk, I will demonstrate a robust toxicant detection platform based on a whole cell sensor array biochip (LumiChip). LumiChip harbors an integrated temperature control and a 16-member sensor array, as well as a simple but highly efficient lens-free bioluminescence collection setup. On LumiChip, samples are infused in an oxygen-permeable microfluidic flow channel to reach the biosensor array. Time-lapse changes in the bioluminescence emitted by the array members are measured on a single linear charge-coupled device (CCD) commonly used in commercial industrial process control or in barcode readers. Removal of the protective window on the linear CCD allowed lens-free direct interfacing of LumiChip to the CCD surface for high numerical aperture measurement. Bioluminescence induced by simulated contamination events was detected within 15 to 45 minutes. The portable LumiSense system utilizing the linear CCD in combination with the miniaturized Lumichip is a promising potential platform for on-site water pollutant monitoring.
Mahnaz M Abdi
University Putra, Malaysia
Title: Conducting polymer composite based on nano-cellulose for biosensing application
Time : 16:40 - 17:00
Biography:
Mahnaz M Abdi received her PhD in Material Chemistry from University Putra Malaysia, 2010. She was with Institute of Tropical Forestry and Forest Products, UPM as a Post-doctoral fellow and continued her career as a researcher at the Luleå University of Technology (LTU), Lulea, Sweden. She currently works as senior lecturer/research associate at University Putra Malaysia. Her research focused on nanocomposite materials from conducting polymers/biopolymers and their application in sensors, biosensors and corrosion protection. Her research is mainly funded by SciencFund and FRGS.
Abstract:
Application of conducting polymers of polypyrrole and polyaniline-cellulose nanocrystal based composite as electron-transfer pathways in enzyme electrodes was investigated. Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane was prepared by chemical polymerization. Modified electrodes were prepared based on drop casting of nanocomposite suspension on the screen printed electrode (SPE) surface following by GOx immobilization. Field emission scanning electron microscopy (FESEM) images showed the porous structure of the nanocomposite with large surface area which could accommodate a large quantity of enzyme and allow the rapid diffusion of the active enzyme into the sensing membrane. The electrochemical and DPV responses of the GOx for glucose biosensor detection were examined in detail. The anodic current (Ip) in the voltammogram of the modified electrode prepared from PPy-CNC showed higher value compare to modified electrode prepared from pure polymer indicating CNC enhanced electron transferring and biosensor performance. The modified glucose biosensor exhibits a high sensitivity (ca. 0.73 μA•mM−1), with a dynamic response ranging from 1.0 to 20 mM glucose. The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples. This sensor displays an acceptable reproducibility and stability over time. The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.