Day 2 :
Keynote Forum
Stephen Trowell
CSIRO, Australia
Keynote: CYBERTONGUE®, a multiplexed biosensing platform where BRET meets flow - for food diagnostic and other applications
Time : 10:00 - 10:30
Biography:
Stephen Trowell completed his PhD in Visual Transduction Biochemistry at the Australian National University. In 1989, he was awarded an Australian National Research Fellowship, which he took up at CSIRO, Australia's National Research Agency, where he obtained tenure as a researcher. He is currently a Senior Principal Research Scientist and Group Leader for Innovative Bio/products. He has authored 54 full papers in international refereed journals and is inventor on 15 patent families. He has served on the Editorial Board of Bioinspiration and Biomimetics since 2010 and is a recipient of CSIROs Newton-Turner career award.
Abstract:
At the same time as the global supply chain for food and food ingredients is diversifying, tolerance for food risk is decreasing and demands for timeliness and cost minimization are intensifying. Current food diagnostic technologies are mainly focused on microbial safety but quality, process ability and chemical composition are increasingly important reasons for testing food. Rather than competing with gold standard analytical methods in accredited centralized testing laboratories, we set out to develop a biosensor platform that can provide rapid, real-time or continuous measurements of a range of different analytes in food, to inform the decisions of food producers and processors. The resulting CYBERTONGUE® technology platform uniquely combines three features. 1) The availability of a diverse repertoire of biosensors, all of which transduce a biochemical event into a photonic signal, using bioluminescence resonance energy transfer (BRET), with profound advantages over competing approaches. 2) The biosensors are used in the fluid phase, i.e., they are not fixed to a surface but mix with the sample on a microfluidic chip and are continuously replenished, and avoiding problems of sensor drift and slow regeneration and 3) Whilst single channel mode is the simplest, we have also demonstrated the use of a multichannel chip with different biosensors in each channel, simultaneously measuring different aspects of a single sample. The same principles can also be applied to biosensors for clinical diagnoses. I will describe the technical basis of CYBERTONGUE® technology and illustrate it with applications to measuring maltose in beer and spoilage proteases in milk.
Keynote Forum
Ruey-an Doong
National Chiao Tung University, Taiwan
Keynote: Amperometric biosensors for cancer maker detection using novel dumbbell-like gold-magnetite nanocomposites
Time : 10:30 - 11:00
Biography:
Ruey-an Doong earned his PhD in Environmental Engineering from the National Taiwan University, Taiwan in 1992. He joined the Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan in 1994. He served as the Department Head in 2008 and Dean of the College of Nuclear Science, National Tsing Hua University in 2011. Currently, he is a Full Professor in the Institute of Environmental Engineering, National Chiao Tung University, Taiwan. His research interest lies in the areas of environmental chemistry and environmental nanotechnology.
Abstract:
An ultrasensitive amperometric immunosensor for the detection of cancer biomarker, ï¡-fetoprotein (AFP), was fabricated using Au/chitosan modified glassy carbon electrode (GCE) and antibody-functionalized dumbbell-like Au-Fe3O4 heterostructures as Biosensing platform and immuno-labels, respectively. To fabricate the labels, nano-Au NPs were first epitaxially grown onto Fe3O4 surface to form the dumbbell-like Au-Fe3O4 followed by conjugation of secondary antibody onto Au surface (Au-Fe3O4-Ab2). Results showed that the GCE modified with chitosan produced high electrochemical response by conjugation of more Au-Ab1 and the dumbbell-like Au-Fe3O4 served as a dual-probe to immobilize Ab2 onto Au as well as to reduce H2O2 by Fe3O4, resulting in the enhancement of signal amplification. The prepared Au-Fe3O4/Ab2/AFP/Ab1/Au/chitosan/GCE immunosensors exhibited a good analytical performance in the presence of 10 mM H2O2 with wide dynamic range of 4 orders of magnitude (0.01–40 ng mL-1) and low detection limit of 2.3 pg mL-1. In addition, the dumbbell-like Pt-Fe3O4 nanoparticles have been used to fabricate the amperometric biosensors for detection of dopamine. The Pt-Fe3O4-based electrode is linearly dependence on dopamine concentration in the range of 10-850 ïM with the detection limits of 0.13-7.22 ïM. Results obtained in this study clearly demonstrate that the dumbbell-like metal-magnetite biosensor is a promising biosensing platform for highly sensitive detection of tumor makers and neurotransmitters.
Keynote Forum
Eunkyoung Kim
Yonsei University, South Korea
Keynote: Photothermal effect of conjugated polymer surfaces for harvesting of live cell sheets
Time : 11:00 - 11:30
Biography:
Eunkyoung Kim has completed her PhD from University of Houston. She is the Director of Active Polymer Center for Pattern Integration (APCPI), an engineering research center of excellence in Korea. She has published more than 170 papers in reputed journals and was awarded the Doctor honoris causa of École Normale Supérieure de Cachan.
Abstract:
The photothermal conversion in conductive polymers has been attracted as the important phenomena to generate heat for bio engineering as well as energy harvesting. In particular, the harvesting of living cell and cell sheet via NIR photothermal effect is of great interest in wide research area including cell therapy, transplantation, tissue engineering and regenerative medicine. In our experiments, the local heating of conductive polymer film or nanoparticles, via NIR photothermal effect of polymers, allowed to stimuli proteins that are interfaced between adherent cells and polymer surface. A collagen layer was coated on a conductive polymer surface prepared from poly (3, 4-ethylenedioxythiophene)s (PEDOT). The NIR photothermal effect of PEDOT surface induced unfolding of collagen triple helices, yielding soluble collagen structures. This dissociation of collagens provided a fast harvesting method of a living cell sheet, within few minutes of NIR irradiation. Using a systematic optical set-up, harvesting of a large area cell sheet and patterned cell sheets were achieved. Effect of the structure and composition of the conducting polymer films on the photothermal conversion as well as harvesting of cell sheets will be discussed.
- Bioelectronics
Biosensor Applications
Biochips & Nucleic Acid Sensors
Biosensing Technologies
Biosensors for Imaging
Photonic Sensor Technologies
Chair
Andreas Hutten
Bielefeld University, Germany
Session Introduction
Stephen Trowell
CSIRO, Australia
Title: CYBERTONGUE®, a multiplexed biosensing platform where BRET meets flow - for food diagnostic and other applications
Biography:
Stephen Trowell completed his PhD in visual transduction biochemistry at the Australian National University. In 1989, he was awarded an Australian National Research Fellowship, which he took up at CSIRO, Australia's national research agency, where he obtained tenure as a researcher. He is currently a Senior Principal Research Scientist and Group Leader for Innovative Bioproducts. He has authored 54 full papers in international refereed journals and is inventor on 15 patent families. He has served on the Editorial Board of Bioinspiration and Biomimetics since 2010 and is a recipient of CSIRO's Newton-Turner career award.
Abstract:
At the same time as the global supply chain for food and food ingredients is diversifying, tolerance for food risk is decreasing and demands for timeliness and cost minimization are intensifying. Current food diagnostic technologies are mainly focused on microbial safety but "quality", process ability and chemical composition are increasingly important reasons for testing food. Rather than compete with gold standard analytical methods in accredited centralized testing laboratories, we set out to develop a biosensor platform that can provide rapid, real-time or continuous measurements of a range of different analytes in food, to inform the decisions of food producers and processors. The resulting CYBERTONGUE® technology platform uniquely combines three features. 1) The availability of a diverse repertoire of biosensors, all of which transduce a biochemical event into a photonic signal, using bioluminescence resonance energy transfer (BRET), with profound advantages over competing approaches. 2) The biosensors are used in the fluid phase, i.e. they are not fixed to a surface but mix with the sample on a microfluidic chip and are continuously replenished, and avoiding problems of sensor drift and slow regeneration and 3) Whilst single channel mode is the simplest, we have also demonstrated the use of a multichannel chip with different biosensors in each channel, simultaneously measuring different aspects of a single sample. The same principles can also be applied to biosensors for clinical diagnoses. I will describe the technical basis of CYBERTONGUE® technology and illustrate it with applications to measuring maltose in beer and spoilage proteases in milk.
Ruey-an Doong
National Chiao Tung University, Taiwan
Title: Amperometric biosensors for cancer maker detection using novel dumbbell-like gold-magnetite nanocomposites
Biography:
Prof. Ruey-an Doong earned his Ph.D. degree in Environmental Engineering from the National Taiwan University, Taiwan in 1992. He joined the Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan in 1994. He served as the Department Head in 2008 and Dean of the College of Nuclear Science, National Tsing Hua University in 2011. Currently, he is a full professor in the Institute of Environmental Engineering, National Chiao Tung University, Taiwan. His research interest lies in the areas of environmental chemistry and environmental nanotechnology.
Abstract:
An ultrasensitive amperometric immunosensor for the detection of cancer biomarker, ï¡-fetoprotein (AFP), was fabricated using Au/chitosan modified glassy carbon electrode (GCE) and antibody-functionalized dumbbell-like Au-Fe3O4 heterostructures as sensing platform and immuno-labels, respectively. To fabricate the labels, nano-Au NPs were first epitaxially growth onto Fe3O4 surface to form the dumbbell-like Au-Fe3O4 followed by conjugation of secondary antibody onto Au surface (Au-Fe3O4-Ab2). Results showed that the GCE modified with chitosan produced high electrochemical response by conjugation of more Au-Ab1 and the dumbbell-like Au-Fe3O4 served as a dual-probe to immobilize Ab2 onto Au as well as to reduce H2O2 by Fe3O4, resulting in the enhancement of signal amplification. The prepared Au-Fe3O4/Ab2/AFP/Ab1/Au/chitosan/GCE immunosensors exhibited a good analytical performance in the presence of 10 mM H2O2 with wide dynamic range of 4 orders of magnitude (0.01–40 ng mL-1) and low detection limit of 2.3 pg mL-1. In addition, the dumbbell-like Pt-Fe3O4 nanoparticles have been used to fabricate the amperometric biosensors for detection of dopamine. The Pt-Fe3O4-based electrode is linearly dependence on dopamine concentration in the range of 10-850 ïM with the detection limits of 0.13-7.22 ïM. Results obtained in this study clearly demonstrate that the dumbbell-like metal-magnetite biosensor is a promising biosensing platform for highly sensitive detection of tumor makers and neurotransmitters.
A P Vladimirov
Ural Federal University, Russia
Title: The use of the speckle dynamics for the quantitative analysis of micro and macro processes in cultured cells: Theory and experiment
Time : 14:30 - 14:50
Biography:
A P Vladimirov graduated from the State University in Cheboksary in 1975. In 1986, he defended his candidate dissertation, and in 2002 the Doctoral dissertation. Since 1972, he has been working in the field of development and application of speckle - holographic methods of measurement and control. He worked at the Institute of Polymeric Materials (Perm) and Institute of Metal Physics (Sverdlovsk). Currently, he is working in three organizations: The Institute of Engineering Science of the Ural branch of the Russian Academy of Sciences, Ural Federal University, and Institute of viral infections (Yekaterinburg).
Abstract:
Previously, the theory of dynamic speckle-interferometry which allows to study intracellular phenomena was developed by the author. The theory has been successfully used by the author with colleagues to study the effects of herpes virus in three types of cultured cells. The purpose of this report is to familiarize colleagues with the specificities of the theory and application of the method. First briefly discusses the theory of the method, the basic formulae used in practice. It is shown that the time-average intensity at the observation point and the correlation coefficient of the area of images depend on the parameters characterizing macroscopic changes (shape of cells) and microscopic processes in cells. The experiment with thawed cells, where the shape changes of cells due to the movement and the passing of intracellular processes is discussed.
Sayed Ahmad Mozaffari
Iranian Research Organization for Science and Technology (IROST), Iran
Title: Preparation and electrochemical characterization of ZnO based disposable urea biosensors
Time : 14:50 - 15:10
Biography:
Sayed Ahmad Mozaffari received his PhD in chemistry under supervision of Professor Reza Karimi Shervedani from University of Isfahan in 2006. Then he undertook Post-Doctoral studies at Pohang University of Science and Technology (POSTECH) in South Korea under supervision of Professor Su-Moon Park (2008–2009). His main areas of interests include electroanalytical chemistry, thin layers and nanotechnology, sensors and biosensors, electrochemistry of solar cells and fuel cells, electrochemical impedance spectroscopy (EIS) and Fourier transform EIS.
Abstract:
Semiconductor based transducers play an important role in electrochemical sensing. Recently, zinc oxide (ZnO) nanostructures have drawn many attentions in the fabrication of biosensors with many advantages including nontoxicity, biological compatibility, fast electron transfer rates, high specific surface area, high catalytic efficiency and easy preparation. In this presentation, nanostructured zinc oxide thin films were prepared by different deposition methods such as sol-gel, RF magnetron sputtering, and electrochemical deposition on the F-doped SnO2 conductive glass (FTO/ZnO-Nano). The effect of deposition technique on the growth and crystallization of ZnO, morphology, thickness and electrical conductivity of ZnO thin film transducer, enzyme immobilization, enzyme activity and stability, and biosensor response will be discussed. EIS technique as a powerful, non-destructive and informative diagnostic tool has become increasingly popular for biosensing applications and is widely used for studying electrical and electrochemical interfacial properties of a large variety of systems. In these studies the electrochemical impedance spectroscopy (EIS) was applied as an assessment method. The fabricated biosensors showed remarkable stability and reproducibility for urea sensing with high sensitivity, low detection limit, broad dynamic range, fast response time and better calibration curve.
Holly Roberts
University of Birmingham, UK
Title: Development of modified ferrocenes DNA probes for electrochemical SNP sensing
Time : 11:45 - 12:00
Biography:
Holly Roberts obtained a 1st Class Hons Masters Degree in Chemistry with Analytical Science from The University of Birmingham in 2013. Her final project was with Dr. J Bunch investigating polymer detection and identification for forensic applications using TOF-SIMS. Later she went on to start a PhD project with Prof. J H R Tucker researching DNA detection using ferrocene modified nucleic acids as an electrochemical reporter for DNA detection. She has won several awards for both poster and oral presentations including Best in Year Oral Presentation and Best Science Poster at a public outreach event. She is also an avid STEM ambassador.
Abstract:
Ferrocene is used regularly as a redox reporter within DNA as it is easily manufactured within the lab, functionalisable, stable and has well defined electrochemical behaviour making it an excellent candidate for DNA sensing. We present a modified ferrocene unit for use as a DNA based surface bound electrochemical probe. Ferrocene modified with a base on each cyclopentadienyl ring has been synthesised and substituted for two of the sugar units in the DNA backbone using automated DNA synthesis. This allows for the unit to be placed as close as possible to the site of a DNA base variation or single nucleotide polymorphism (SNP) thus increasing the sensitivity of the probe. An FcNA octomer has also been synthesised and investigated using cyclic voltammetry with a view to using the functionalised ferrocene to produced nanowires and redox active nanomaterials. On a gold electrode, surface formation gives reproducible redox behaviour both single stranded and upon hybridisation. Investigations using Square Wave Voltammetry (SWV) suggest an unusual increase in current on binding to a complementary DNA strand due to the effect of increased molecular rigidity. There is also some variation between fully complementary and partially non-complementary DNA strands which shows early promise for SNP detection. The probe has shown good chemical and electrochemical stability, losing only 2% current signal over 150 scans. The ability to sense SNPs using an electrochemical group closely associated to the site of interest could give a sensitive, portable, reusable and rapid SNP detector leading to faster medical diagnosis.
NaHyun Cho
Stony Brook University, USA
Title: Ordered DNA fragmentation using soft lithography and amplification for next generation sequencing
Time : 12:00 - 12:15
Biography:
NaHyun Cho is a PhD candidate in Materials Science and Engineering at Stony Brook University in New York. She previously earned a BS in Engineering Chemistry and a ME in Materials Science and Engineering from at Stony Brook University. She spent two and half years in the field as a research engineer with LG Electronics in Seoul, South Korea, working in their crystalline solar cell R&D Center. Her current area of research is ordered DNA fragmentation on surfaces for next-generation sequencing. In order to further develop her research focus, she is a visiting student at both Cold Spring Harbor Laboratory and Brookhaven National Laboratory in New York.
Abstract:
Current Next Generation Sequencing (NGS) technology starts with randomly fragmented DNA from whole genomic DNA. Because of this randomness, all DNA fragments need to sequence massive parallel reads in order to know the whole sequencing. In this study, we try to cut DNA fragments into 10-15 kbps using soft lithography technology because one of the NGS platforms is Pacific Biosciences’ RS, able to read larger size fragments, up to 15 kilo base pairs, quickly. Also, as an ultimate goal, we will try to keep the DNA fragments in the orders from the surface, so the DNA reads do not need to be sequenced several times. In previous studies, we stretched DNA on PMMA (Poly Methyl Methacrylate) substrate and the stretched DNA could be linearly cut with soft lithography by applying DNase I enzyme. After cutting the DNA on the substrate, in order to sequence the DNA fragments with NGS technology, the DNA fragments are taken from the surface and placed in a solution base. We dissolved the PMMA substrate and fragmented DNA fragments together and separated the DNA fragments using a Phenol-Chloroform Isoamyl (PCI) extraction procedure. The principle of separating DNA with PCI mixture is based on solubility differences between organic and aqueous liquids. DNA is a negatively charged, hydrophilic bio-polymer because of its negatively charged phosphate groups. On the other hand, PMMA is a non-charged polymer that is dissolved in chloroform. By dissolving the PMMA surface, it is possible to separate DNA from the surface using liquid-liquid phase separation (Organic phase: Aqueous phase). For the data processing, confocal microscopy was used to take images of cut DNA on the PMMA surface. Gel electrophoresis and bioanalyzer were conducted to confirm the distribution of the DNA fragments. Finally, PacBio RS II which is the one of the long-read Next-Generation sequencing platforms was used to confirm quality and quantity of the fragmented DNA from surfaces.
Gautam Anand
Auckland University of Technology, New Zealand
Title: Parametric electrical modelling of human forearm simulation response using multi-frequency electrical bioimpedance
Time : 12:15 - 12:30
Biography:
Gautam Anand is a PhD researcher at Institute of Biomedical Technologies, AUT University. With a background in Electrical and Electronics Engineering, his research interests include biophysics, biosignal acquisition, bioelectronics, bioimpedance analysis and its applications in medical diagnostics.
Abstract:
This work presents a parametric electrical modelling of the electrical response of human forearm tissues through a simulation of Multi-frequency Electrical Bioimpedance analysis (MF-EBIA). The objective is to estimate the resistance and capacitance values of the three tissue domains in the forearm – the fat, muscle and artery, through parametric analysis. Following up from a simulation analysis of the human forearm model using Ansys® High Frequency Structure Simulator (HFSS), this work assumes an electrical analogy of the human forearm section for every tissue and calculates the electrical parameters. The tissue model was considered to be isotropic with regards to the dielectric properties and the consideration of blood flow was realised by taking three instances of radial artery diameter. The obtained values of resistance and capacitance for every tissue domain provide an insight into their significant contribution to the overall electrical response, which can be important while analysing their individual electrical behaviour and also helpful in various pre-experimental studies related to dielectric characterization of living tissues.
Maritza P Garrido
University of Chile, Chile
Title: Role of sympathetic nervous system in rat ovarian ageing
Time : 12:30 - 12:45
Biography:
Maritza Garrido is a young academic researcher who works in Endocrinology and Reproduction Biology Lab of the Jose Joaquin Aguirre Clinical Hospital, pertaining to the University of Chile. She collaborates with the Medicine, Chemical Sciences and Pharmaceutics Schools of the same University, teaching and participating in the development research projects. She is Biochemist and MSc from the same university and currently attends her Pharmacology PhD studies in the same institution.
Abstract:
At present, women have delayed motherhood after 30 years. From this age, the problems of fertility arise and the success percentage of in vitro fertility programs declines. Then, it is important to know the mechanisms involved in the ovarian aging in this period. One of the mechanisms involving in the ovarian aging is the increased sympathetic nerve activity (SNA). In turn, it has been described that the ovary of women post menopause has a higher density of nervous fibers as compared with age-matched control women. We have demonstrated that a rat reproductive aging has also an increased ovarian SNA, which is strongly correlated with the spontaneous appearance of follicular cysts, decreased ovulation and loss of preantral follicles in the ovary. The relevance of these findings was that they have a direct clinical application. The adrenergic receptors present in the ovary are mostly of the β2-adrenergic receptors subtype and noradrenaline exert their effect through them, modifying follicular development and steroid secretions being an increased activity associated with formation and maintenance of cystic structures. Blockade of β-adrenergic receptors recovered ovarian function during reproductive aging. Besides, the successful use of electro acupuncture procedures (decrease SNA) both in rats and patients permit the recovery of ovulatory cycles and ovarian function. The highest sympathetic innervation found in postmenopausal women suggests that have a correlation between the infertility by ageing and by activation of sympathetic nerves. The control of the sympathetic activity could expand out of the window of subfertility and it could promote successful fertility program in ageing woman.
Ling Zhang
Southeast University, China
Title: DMSA-coated iron oxide nanoparticle greatly affects the expression of genes coding cysteine-rich proteins by its DMSA coating
Time : 12:45 - 13:00
Biography:
Ling Zhang was born in HuBei province, china; date of birth: 23/6/1980. Educational background includes: Ph.D candidate in Biomedical engineering, State Key Laboratory of Bioelectronics, school of biological science and medical engineering, Southeast University, Nanjing, PR China. She is mainly interested in study on biological effects of iron oxide nanoparticles (FeNPs) and bioinformatics.
Abstract:
The dimercaptosuccinic acid (DMSA) was widely used to coat iron oxide nanoparticles (FeNPs); however, its intracellular cytotoxicity remains to be adequately elucidated. This study analyzed the differentially expressed genes (DEGs) in four mammalian cells treated by a DMSA-coated magnetite FeNP at various doses for different times. The results revealed that about one fourth of DEGs coded cysteine-rich proteins (CRPs) in all cells under each treatment, indicating that the nanoparticles greatly affected the expressions of CRP-coding genes. Additionally, about 26% of CRP-coding DEGs were enzyme genes in all cells, indicating that the nanoparticles greatly affected the expression of enzyme genes. Further experiments with the nanoparticles and a polyethyleneimine (PEI)-coated magnetite FeNP revealed that the effect mainly resulted from DMSA carried into cells by the nanoparticles. This study thus firstly reported the cytotoxicity of DMSA at the gene transcription level as coating molecules of FeNPs. This study provides new insight into the molecular mechanism why the DMSA-coated nanoparticles resulted in the transcriptional changes of many CRP-coding genes in cells. This study evokes attention toward the intracellular cytotoxicity of DMSA as coating molecule of nanoparticles, which has very low toxicity as orally administered antidote due to its extracellular distribution.
Anubha Kalra
Auckland University of Technology, New Zealand
Title: Quantifying skin stretch induced motion artifact from an electrocardiogram signal
Time : 13:00 - 13:15
Biography:
Anubha Kalra is curently a Doctoral candidate at Institute of Biomedical Technologies (IBTec), AUT University. Her research interests include ambulatory ECG monitoring and analysis, artifact detection, adaptive analysis for artifact reduction and biosignal data filtering and acquisition
Abstract:
This work presents a 2D quantification of strain field caused due to the motion artifact in an Electrocardiogram (ECG) measurement. The objective of this work is to estimate the skin stretch induced motion artifact in an ECG signal. An ECG measurement was obtained from a subject for 10 seconds using standard Ag/AgCl electrodes by continuously moving the arm back and forth during the measurement. The motion artifact produced due to the arm movement was emulated using a Poly dimethyl siloxane (PDMS) patch of dimensions 40mm x 45mm x 0.254mm adhered to the arm. The movement of the PDMS patch during the ECG measurement was recorded in a video and motion artifact was quantified in terms of normal and shear strain components εx, εy and εxy. These values were derived using feature detection and Euclidean distance feature mapping. The obtained motion artifact can be eliminated from the ECG signal using adaptive filtering or other techniques such as Extended Kalman Filtering (EKF). This method of evaluation of the strain components was validated against a finite element analysis SolidWorks®.
Everson Thiago Santos Geroncio da Silva
State University of Campinas, Brazil
Title: Triboelectric effect as a novel tool for the development and application of point-of-care testing devices
Time : 14:00 - 14:15
Biography:
Everson Thiago Santos Geroncio da Silva has completed his PhD from State University of Campinas, Brazil. Presently, he is working as a Postdoctoral Researcher, where he is responsible for the area of development and application of point-of-care testing devices and conductive inks. He is the first author of the paper published in Lab-On-A-Chip journal (2015) regarding the use of triboelectric effect for sealing and controlling the flow in paper-based devices.
Abstract:
Triboelectric effect (TEE) is a theory related to contact electrification (tribocharging), which happens when two materials are brought into contact (by rubbing, for example) and then separated, creating electrostatic charges. The accurate mechanism of the charge formation is still a motive for debate and not completely understood, but these tribocharged materials may be useful as electrostatic masks for the construction of paper-based analytical devices (μPADs), besides being able to promote reversible electrostatic sealing in μPADs, which is of great importance in this type of device. PADs are an emerging class of point-of-care (POC) technology that has gain much attention in the past few years due to its simplicity and low cost. Despite the numerous advantages of this substrate, most of POC devices fabricated using this platform have the limitation of being directly exposed to ambient conditions, which may increase the risk of contamination and evaporation of the solvent/sample used in the analysis, proving the importance of sealing. The charged surfaces can also attract aqueous solution, delaying the fluid delivery and minimizing its evaporation. In summary, the main goal of this presentation is to show the possibilities of using charged surfaces as a versatile tool for the development of μPADs and other POC devices.
Shengwei Tan
Southeast University, P R China
Title: Sequence-specific recognition of DNA oligomer by DNA/DNA hybridization in silicon nitride nanopores
Time : 14:15 - 14:30
Biography:
Shengwei Tan obtained her PhD in Biomedical Engineering from State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, P R China in 2016, MESc in Applied Chemistry from College of Chemistry and Chemical Engineering, Guangxi University for Nationalities, P R China in 2011 and BE in Chemical Engineering and Technology from Department of Chemistry, Dezhou University, P R China. Prior to registering for PhD, she was engaged in the scientific research work at Zhejiang University, Hangzhou, PR China, and the main research interest was in Analytical Chemistry.
Abstract:
Nanopores have been proven to be novel and versatile single-molecule sensors for individual unlabeled biopolymer detection and characterization. In the present study, a relatively large silicon nitride (Si3N4) nanopore with a diameter of approximately 60 nm was fabricated successfully using a focused Ga ion beam (FIB). We demonstrated a simple ex situ silanization procedure to control the size and functionality of solid-state nanopores. The presented results show that by varying the silanization time, it is possible to adjust the efficiency of probe molecule attachment, thus shrinking the pore to the chosen size, while introducing selective sensing probes. The functionalization of nanopores was verified by analysis of field-emission scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDS), and electrical measurements. Based on this study, we envision that the functionalized silicon nitride nanopores with the DNA probe might provide a biosensing platform for the detection and discrimination of a short single-stranded DNA oligomer of unknown sequences in the future.
Greter Amelia Ortega RodrÃguez
Center for Applied Science and Advanced Technology of IPN, Mexico
Title: Magnetite nanoparticles on paper: A platform for the diagnosis of dengue fever by magnetic -ELISA
Biography:
Greter Amelia Ortega Rodríguez has completed her Bachelor of Science in Chemistry (Summa cum Laude) and her Master of Science in Chemistry at University of Havana, Cuba in 2012 and 2015, respectively. At present, she is a graduate teaching assistant at the Department of Inorganic Chemistry, Faculty of Chemistry, University of Havana and a PhD student at Center for Applied Science and Advanced Technology of IPN, Legaria Unit, Mexico. She has participated in 10 international scientific meetings held in Cuba and has done research on synthesis, functionalization and the use of the novel properties of metal and magnetic nanoparticles to design nanostructured biosensors.
Abstract:
Enzyme-linked immunosorbent assays (ELISAs) are the most widely used methods to detect antibodies. However, they have some drawbacks. As a result, in recent years magnetic nanoparticles or beads (e.g. magnetites) have been combined with ELISAs to improve their analytical performance. On the other hand, despite paper-based ELISA are less sensitive than conventional ELISA, they emerge as suitable platforms to develop disposable devices for point-of-care diagnostic. A novel “magnetic-ELISA”, based on core-shell magnetite@polydopamine nanoparticles supported on Whatman paper was developed to detect IgM-dengue antibodies. An affordable procedure to deposit magnetite nanoparticles on paper surfaces (Whatman thype-1 and Whatman thype-ss903) and, to conjugate such nanoparticles with Anti human-IgM antibodies using polydopamine as linker, is reported. Structural features, magnetic behavior, coating homogeneity, as well as, the nanoparticles/linked antibodies ratio were determined. The analytical performance of “magnetic-ELISA” supported on paper surface was 100 times more sensitive with a 700 times lower limit of detection than traditional ELISA or using magnetic beads without depositing on paper to detect IgM-dengue antibodies. Additionally, the new system showed low background, acceptable reproducibility, low-cost, easy manufacturing and effortless and easy handling which are very important, considering the large number of biological samples to be processed by a laboratory in case of dengue epidemics.
Ching-Hwa Cheng
Feng-Chia University, Taiwan
Title: The image guiding system for minimally invasive surgery
Biography:
Ching-Hwa Cheng received an MSEE degree from Chung Hwa University, Taiwan, in 1993 and a PhD degree in Computer Science and Information Engineering from National Chung Cheng University, Taiwan, in 2000. His PhD dissertations and Master’s thesis are focused on logic optimization and fault tolerant computing, respectively. He is currently a full professor of Electronic Engineering at Feng-Chia University. His research interests include endoscope system design, image process and low-power VLSI Design/EDA/testing related issues, such image guiding surgery and low-power chip designed by multi-voltage.
Abstract:
The endoscope is widely used for various diagnoses and treatments in Minimally Invasive Surgery (MIS), such as hysteroscopy, laparoscopy and colonoscopy. However, the limited field of image of the endoscope is often the most problematic issue faced by surgeons and medical students, especially for those inexperienced physicians, which leads to difficulty during surgical operations. To reduce the difficulties of MIS with respect to endoscope function, the proposed technique provides the angle and distance from the surgical instrument to the lesion. The in-time guiding information provides global positioning information by tracking the lesion position during surgery. The whole system has been successfully validated in animal in vivo experiments. As the view of the scope is limited by the endoscope, and when the surgical instrument moves, the lesion and instrument relating location information is lost. As the global position information is lost, the surgical risk increases. Hence, the proposed technique of this current research provides the angle and distance from the surgical instrument to the lesion. This can help the doctor obtain the needed global positioning information. The risk situation occurs when the surgical three-dimensional positioning information is lost. Hence, the image guiding requirement is urgently needed for increased precision in surgical operations, such as in heart and brain surgeries. The proposed in-time image guiding technique applies to the HSV object-tracking method. This technique provides the distance and angle information in order to help doctors perform successful surgical operations. The guiding information prevents the surgical errors from occurring due to the limited image of field of the endoscope. The proposed technique can also be adopted to decrease the training cost of new doctors. During a survey of the previously published researches, to our knowledge, there are no similar works in image guiding for MIS.
Ajay Giri Prakash
Stanford University School of Medicine, USA
Title: Bioinspired smart skin of self-powered hair cell sensors for ultrasensitive flow sensing
Biography:
Ajay Giri Prakash Kottapalli is working as a Post-doctoral Associate at the Center for Environmental Sensing and Modeling (CENSAM) interdisciplinary research group (IRG) of Singapore-MIT Alliance for Research and Technology (SMART). His research interests include biomimetics, bio-inspired MEMS/NEMS sensors, soft-polymer sensors, biomaterials, nature inspired sensors, piezoelectric actuators etc. He has published more than 15 international papers in reputed journals and about 40 international conference proceeding papers. He filed over 4 national wide patents and has been serving as an editorial board member of a wide-range of well-reputed journals.
Abstract:
Ajay Giri Prakash Kottapalli is working as a Post-doctoral Associate at the Center for Environmental Sensing and Modeling (CENSAM) interdisciplinary research group (IRG) of Singapore-MIT Alliance for Research and Technology (SMART). His research interests include biomimetics, bio-inspired MEMS/NEMS sensors, soft-polymer sensors, biomaterials, nature inspired sensors, piezoelectric actuators etc. He has published more than 15 international papers in reputed journals and about 40 international conference proceeding papers. He filed over 4 national wide patents and has been serving as an editorial board member of a wide-range of well-reputed journals.
Zhiyuan Shen
Singapore-MIT Alliance for Research and Technology, Singapore
Title: Biomimetic flow sensors for biomedical flow sensing in intravenous tubes
Biography:
SHEN Zhiyuan worked as a research staff at Institute of Materials Research and Engineering (IMRE), Singapore. He is currently a postdoctoral associate at the Center for Environmental Sensing and Modeling (CENSAM), SMART, Singapore. Zhiyuan was awarded Ph.D. in mechanical engineering from Nanyang Technological University (NTU), Singapore, 2013. He obtained his M.E. and B.E., both in microelectronics and solid state electronics, from Xi’an Jiaotong University (XJTU), China in 2008 and Harbin Institute of Technology (HIT), China in 2005. His research interests include MEMS sensors and actuators, piezoelectric materials and devices, acoustic and ultrasonic transducers, ultrasonic NDT, biomimetic flow sensors, and metamaterials.
Abstract:
A MEMS piezo-resistive flow rate sensor inspired by the hair cell sensor found in the fish lateral line system has been composed and fabricated. The sensor has been bonded with a 3D printed fixture and they together have been integrated on an intravenous tube. The responses of the sensor with respect to two flow rates of 200mL/h and 500mL/h have been tested using a experimental setup, the flow rate in which has been controlled by a peristaltic pump. The sensor shows distinguished response difference with respect to these two flow rates and can be used to monitor the flow rate in the IV tube in the future.
Pouya Rezai
York University, Canada
Title: Integrated hybrid biosensors for automated manipulation and drug screening of whole biological organisms of human diseases
Biography:
Pouya Rezai is an emerging researcher in the areas of microfluidic and biosensors. He is the Graduate Program Director of the Department of Mechanical Engineering at York University. He received his PhD in Mechanical Engineering (Bio-Microfluidics) from McMaster University in Canada. He was an NSERC Visiting Fellow at Public Health Agency of Canada before joining York University in July 2013. He has published over 40 scholarly articles in the format of journal papers, peer-reviewed conference papers, book chapters and patents. His research interests are Microfabrication, Microfluidics, Lab-on-Chips and Bio-Micro-Electro-Mechanical Systems for biology, medicine and environmental science applications.
Abstract:
Small vertebrate and invertebrate organisms such as Danio rerio, C. elegans and D. melanogaster are widely used as model organisms to answer fundamental biological questions about human diseases and to screen chemicals against them for finding new therapeutic drugs. Many biological assays and investigations on these organisms are still conducted manually by laboratory personnel who must possess high levels of expertise in particular unit operations and have access to highly specialized equipment to conduct the assays. Therefore, controllable and sensitive biological studies cannot be performed at the point-of-care or the point-of-need, resulting in significant negative impacts on the outcomes of these researches. In my talk, I will introduce you to bio-microfluidics, a domain of bioengineering research that aims at developing miniaturized and automated devices for efficient biological studies in a cost-effective and sensitive manner. The focus of my talk will be placed on microfluidic chips for studying neurological and behavioral responses of whole-organisms to external cues. Examples include microchips for cardiac and central nervous system studies on Drosophila melanogaster larvae, correlating neuro-behavioral responses of C. elegans to electrical signals, and adult Drosophila egg-laying assays on chemically- and physically-controllable substrates. Since development of these microchips is highly dependent on materials with sensing and actuation functionalities and their transformation into microstructures in the devices, I will also introduce our recent activities in developing conductive polymeric microstructures in microchannels for sensing and detection applications.
Anju Joshi
Indian Institute of Technology, India
Title: Non-enzyamtic electrochemical sensing of hydrogen peroxide using (TBA)3[PMo12O40]-OCNTs composites
Biography:
Anju joshi has completed MTech (Nanotechnology) from University of Rajasthan (Jaipur) in year 2102. Afterwards, she started the pursuit of her PhD under the guidance of Dr. Tharamani C.N. Her research interest focuses on development of novel sensors for more effective determination of biomolecules associated with diseased state.
Abstract:
Nanocomposites based on (TBA)3[PMo12O40]@oxidized carbon nanotubes (O-CNTs) has been prepared to demonstrate its application for hydrogen peroxide sensing. To achieve the desired goal, a keggin type tetra-butylammonium phosphomolybdate has been engrafted onto oxidized carbon nanotubes utilizing electrostatic interactions between oxidized carbon nanotubes and (TBA)3[PMo12O40]. The synthesis procedure is convenient, rapid and scalable (TBA)3 [PMo12O40]- O-CNTs modified GCE showed good stability and well resolved intense redox peaks corresponding to the PMo12 system. Furthermore, cyclic voltammetric, chronoamperometry and rotating disc electrode studies has been performed to evaluate the superior electrocatalytic activity of the (TBA)3[PMo12O40]@O-CNTs modified GCE towards electrocatalytic reduction of hydrogen peroxide. (TBA)3[PMo12O40]@O-CNTs exhibits superior sensitivity, selectivity and fast response with a wide linear range of 1µM-1000 µM.