Day 1 :
Keynote Forum
Dr.Mahmoud F. Almasri
"1Department of Electrical and Computer Engineering, University of Missouri, Columbia.MO 2Department of Co-operative research, Lincoln University, Jefferson City, MO"
Keynote: An Impedance Biosensor for Rapid Detection of Low Concentration of Escherichia coli O157:H7
Biography:
Mahmoud Almasri received BSc and MSc degrees in physics from Bogazici University, Istanbul, Turkey, in 1995 and 1997, respectively, and a PhD in electrical engineering from Southern Methodist University (SMU), Dallas, TX, in 2001. He is currently an associate professor with the Department of Electrical Engineering and Computer Science, University of Missouri. From 2001 to 2002 he was a research scientist with General Monitors, Lake Forest CA. From 2002 to 2003 he was with College of Nanoscale Science and Engineering Albany, NY, as a post doctoral research associate, and from 2004 to 2005 he was with Georgia Institute of Technology as a post doctoral fellow, and a research scientist. His current research include impedance biosensors, MEMS capacitors for power harvesting, Si-Ge-O infrared material, metasurface based uncooled IR detectors, and MEMS Coulter counter for studying time sensitive cell. His research is funded by agencies such as NSF, USDA, ARO, Leonard Wood Institute, and Coulter Foundation.
Abstract:
This presentation will provide an overview of the food safety testing requirements for ready to eat (RTE) food, and raw (NRTE) food, and will discuss the recent impedance biosensor developments in my group for rapid and simultaneous detection of single and multi-pathogens in poultry. The device initially focuses and concentrates the bacteria into the centerline of the microchannel, and directs them toward the sensing region. The bulk media will be directed to the waste outlets through the outer channel. The bacteria will then be trapped on top of the sensing region using trapping electrodes which confine and facilitate the contact and binding of salmonella antigens with salmonella antibody immobilized on the detection electrodes. Various low concentration E.coli and Salmonella samples were tested with and without the trapping electrodes to determine the sensitivity of the biosensor. The lowest measured concentration of Salmonella cells was found to be 13 cell/ml with a detection time of 30 minutes
Keynote Forum
Dr. Manh-Huong Phan
Department of Physics, University of South Florida, Tampa, Florida 33620, USA
Keynote: Recent Developments in Magnetic Impedance Biosensors and Related Medical Devices
Time : 10:10 AM
Biography:
Manh-Huong Phan obtained a global education with B.S., M.S., and Ph.D. degrees in Physics from Vietnam National University (2000), Chungbuk National University – South Korea (2003), and Bristol University – United Kingdom (2006), respectively. He is an Associate Professor of Physics at the University of South Florida. He has published more than 230 peer-reviewed journal papers (h-index: 37 from Google Scholar) and 1 text book. He is an Associate Editor for the Journal of Electronic Materials and the Managing Editor for the Journal of Science: Advanced Materials and Devices."
Abstract:
Early detection of cancer cells in the body greatly increases the chances of successful treatment. While traditional methods, such as visual identification of malignant changes, cell growth analysis, specific-ligand receptor labeling, or genetic testing often require lengthy analysis, a combination of ultrasensitive magnetic field sensors with functionalized magnetic nanoparticles offers a promising approach for a highly sensitive, simple, and quick detection of cancer cells and biomolecules. In this talk, I will review recent progress in the development of magnetic impedance biosensors using nanoparticles. I will present a new approach that integrates the magneto-resistance (MR), magneto-reactance (MX), and magneto-impedance (MI) effects [1] to develop a functional magnetic biosensor with tunable and enhanced sensitivity [2]. The MX-based probe shows the most sensitive detection of superparamagnetic nanoparticles (~10 nm diameter) at low concentrations. A novel biosensor based on the MX effect of a soft ferromagnetic ribbon with a microhole-patterned surface has been developed, demonstrating its high capacity for the detection and quantification of anticancer drugs and proteins tagged to Fe3O4 nanoparticles, as well as Lewis lung carcinoma (LLC) cancer cells that have taken up Fe3O4 or MnO nanoparticles [2,3]. Finite Element simulation fully supports the experimental observations. Finally, novel classes of magnetic nanostructures for advanced biosensing [4,5] and new exploration in medical diagnostics [6] will be discussed.
Keynote Forum
Dr.David W. Schmidtke
Department of Bioengineering, University of Texas at Dallas, Richardson, TX, 75080, USA
Keynote: Novel Redox Polymer Films for Biosensing and Biofuel Cell Applications
Time : 11 AM
Biography:
Dr.David W. Schmidtke is a Professor of Bioengineering at the University of Texas at Dallas (UT-Dallas). Dr. Schmidtke received his Ph.D. in Chemical Engineering from the University of Texas at Austin and completed his postdoctoral studies in the Institute of Medicine and Engineering at the University of Pennsylvania. Prior to joining UT-Dallas, Dr. Schmidtke was a Professor of Chemical Engineering at the University of Oklahoma, and served as the Director of the University of Oklahoma Bioengineering Center. Dr. Schmidtke has been a recipient of both an American Heart Association Scientist Development Award and a National Science Foundation CAREER Award.
Abstract:
Molecular wiring of the redox centers of enzymes to electrode surfaces via redox polymers has attracted considerable attention due to its use in developing biosensors for metabolic monitoring of glucose in diabetes, detection of hybridization reactions in RNA and DNA assays, antigen-antibody binding in immunoassays, and in miniaturize biofuel cells. However for these devices to be useful their sensitivity and lifetime must be sufficient for them to be operated by portable low-cost electronics. This talk will describe our research on the design of a new class of redox polymers based on attaching ferrocene (Fc) redox centers to linear polyethylenimine (LPEI). We will provide an overview of how the polymer and redox center structure affects their stability, redox potential, and ability to electrically communicate with enzyme redox centers. We will discuss how these novel redox polymers can electrically communicate with the redox centers of a variety of enzymes (e.g. glucose oxidase, horseradish peroxidase, fructose dehydrogenase) and generate bioelectrocatalytic current densities > 1 mA/cm2. Finally we will discuss how these redox polymers can be combined with the unique properties of Single-Walled Carbon Nanotubes (SWNTs) for both biosensing and enzymatic biofuel cell applications.
Keynote Forum
Dr.Rosa F Dutra
Federal University of Pernambuco, Brazil
Keynote: Electrochemical and label-free immunosensors applied to point-of-care testings
Time : 11:30 Am
Biography:
Dr.Rosa F Dutra is a Professor in Department of Biomedical Engineering at Federal University of Pernambuco. She is an Electronic Engineer, completed her Master’s in Biomedical Engineering, PhD and Post-doc in Biotechnology. Her research is focused on immuno-sensors, geno-sensors, biomimetic sensors, surface modification, synthesis and functionalization of nanomaterials developing several platforms for measuring biomolecules interaction, using acoustic, optical and electrochemical transducers, focusing in the point-of-care testing. She has supervised more than 25 Post-graduate students and nowadays, she coordinates four great health research projects
Abstract:
Point-of-care testings hold great promise for economic, rapid and practical diagnosis of several diseases, benefiting those with high morbimortality rates. Electrochemical biosensors using amperometric transduction have several advantages as compared to optical and acoustical transductions due to their ease scale up, fast response and low cost connection with compact (hand-held) analyzers. However, several drawbacks on performance have limited their large use in clinical diagnostic. The purpose of this study is to describe some strategies of sensor platforms using conducting polymer integrating carbon allotropes including carbon nanotubes, graphene and fullerene, in order to overcome challenges required as a low limit of detection and a good reproducibility.