Hong-Yu Yu
South University of Science and Technology, China
Title: A novel leaky surface acoustic wave (LSAW) biosensor for label-free detection of hepatitis B surface antibody (HBsAb)
Biography
Biography: Hong-Yu Yu
Abstract
As a potential biochemical sensing platform, leaky surface acoustic wave (LSAW) biosensor consists of a special orientation of a piezoelectric crystal and shows superb sensitivity, speed and reliability. In this work, a novel label-free LSAW biosensor for detecting hepatitis B surface antibody (HBsAb) is fabricated. The sensing area of LSAW is coated with 500nm thick gold membrane on a 100MHz LiTaO3 piezoelectric single crystal. Then the hepatitis B surface antigen (HBsAg) is immobilized on the surface of a gold electrode. The phase shift of LSAW is monitored to detect HBsAb, which increases the mass loading of LSAW by binding to immobilized HBsAg. A reference LSAW device is immobilized with bovine serum albumin (BSA) to block binding of HBsAb. This sensor shows great specificity and sensitivity in detecting HBsAb.