Biosensors & Bioelectronics

Biography

Biography: Sanghamitra Mandal

Abstract

This paper describes the fabrication of a glucose sensing biosensor.The working principle is based on the electrochemical reaction taking place between immobilized glucose oxidase adsorbed by the ZnO nanorods, and the electrolyte glucose.Zinc oxide (ZnO) nanorods were synthesized on indium titanium oxide coated glass substrate using the hydrothermal sol-gel growth technique. Characterization of the ZnO nanorods is performed using the absorption spectroscopy, micro-Raman spectroscopy and scanning electron microscopy. A blue shift is observed in the optical band gap due to increased particle size and density of the ZnO nanorods compared to bulk ZnO.The two-electrode system is employed to measure current for sensing the glucose concentration inside an electrochemical cell. Nafion/GOx/ZnO nanorods/ITO is used as the working electrode, and platinum plate as the reference electrode. Amperometric response for clinical range of blood glucose concentration from 0.25 20 mM is measured at 0.8 V. The response time for the proposed biosensor is identified to be less than 3 s. For a linear range of glucose concentration from 0.25 7.5mMthe analyzed sensitivity is 51.3 µA/cm2 mM-1,and the lower detectionlimit is5 µM. The electrochemical characterization of the biosensor is performed using the cyclic voltammetry method for a voltage range from -0.2 1 V at a scan rate of 0.1 V/s. The achieved results indicate that ZnO nanorods based working electrode demonstrate much higher current response, excellent stability, reusability, and faster response time than previously reported enzymatic electrochemical biosensors.