Dr.Mahmoud F. Almasri
"1Department of Electrical and Computer Engineering, University of Missouri, Columbia.MO 2Department of Co-operative research, Lincoln University, Jefferson City, MO"
Title: An Impedance Biosensor for Rapid Detection of Low Concentration of Escherichia coli O157:H7
Biography
Biography: Dr.Mahmoud F. Almasri
Abstract
This presentation will provide an overview of the food safety testing requirements for ready to eat (RTE) food, and raw (NRTE) food, and will discuss the recent impedance biosensor developments in my group for rapid and simultaneous detection of single and multi-pathogens in poultry. The device initially focuses and concentrates the bacteria into the centerline of the microchannel, and directs them toward the sensing region. The bulk media will be directed to the waste outlets through the outer channel. The bacteria will then be trapped on top of the sensing region using trapping electrodes which confine and facilitate the contact and binding of salmonella antigens with salmonella antibody immobilized on the detection electrodes. Various low concentration E.coli and Salmonella samples were tested with and without the trapping electrodes to determine the sensitivity of the biosensor. The lowest measured concentration of Salmonella cells was found to be 13 cell/ml with a detection time of 30 minutes