Shalini Menon*, K. Girish Kumar
Cochin University of Science and Technology, INDIA
Title: A dual channel optical sensor for norepinephrine via insitu generated silver nanoparticles
Biography
Biography: Shalini Menon*, K. Girish Kumar
Abstract
Norepinephrine (NE) is considered as one of the naturally occurring catecholamines which acts both as a hormone and a neurotransmitter. Catecholamine levels are used for the diagnosis and regulation of phaeochromocytoma, a neuroendocrine tumor of the adrenal medulla. The development of simple, rapid and cost-effective sensors for NE still remains a great challenge. Herein, a dual channe l sensor has been developed for the determination of NE.
A mixture of 10 mL of 1.00×10-2 M AgNO3, 0.5 mL of 1.00×10-2 M NaOH, 0.2 mL of 0.5% NH3.H O and 0.5 mL of 0.02 % cetrimonium bromide (CTAB) working solution was prepared. The mixture was vortexed thoroughly, and to 2 mL of this solution an appropriate volume of 1.00×10-5 M NE solution was added. Two minutes later, the absorption and fluorescence intensities were measured against the reagent blank solution treated in the same way without NE. Fluorescence intensities were recorded by exciting at a wavelength of 400 nm.
A colorimetric and fluorometric dual channel sensor has been fabricated for the determination of NE. Visual detection is possible due to the formation of brown silver nanoparticles (NPs) in the presence of NE, which further resulted in strong metal enhanced fluorescence signals. A linear relationship was obtained between the absorbance values and concentration of NE in the range 1.00×10-6 M- 6.66×10-8 M; the detection limit being 1.79×10-8 M. It was also found that the fluorescence intensities were proportional to the concentration of NE over the range of 8.92×10-3 M- 5.66×10-5 M, with the corresponding limit of detection 5.59×10-6 M. Furthermore, application of the present approach in synthetic blood serum has been demonstrated, which suggests its great potential for diagnostic purposes.