Seongjae Jo
Korea University, South Korea
Title: Detection of cortisol using aptamer functionalized gold nanoparticles based on localized surface plasmon resonance
Biography
Biography: Seongjae Jo
Abstract
Depressive disorder is one of the most common mood disorders and is induced by a variety of factors including neurobiological changes, combinations of interacting genes, the environment, or the life cycle of an individual. Generally, mental health or psychological tests are performed to identify depressive disorders. These method can lead to inaccurate results due to differences in patient's personal preferences and responses. So it is therefore difficult to analyze between depression and psychological reasons. Cortisol is a well-known biomarker of depressive disorder. By measuring cortisol in human body, we can diagnose their condition more accurately. In this study, we measure the cortisol using aptamer functionalized gold nanoparticles substrate based on localized surface plasmon resonance. We synthesize gold nanoparticles of various sizes to find the optimal conditions for aptamer functionalized substrates and use them to make substrates. The detection limit (LOD) is 0.1 nM and also has excellent selectivity for steroid hormones whose molecular weight and structure are similar to cortisol. In addition, we measured cortisol from real saliva and we compared the result with ELISA for significant results.