Ali A Ensafi
Isfahan University of Technology, Iran
Title: Layer-by-layer electrochemical and spectroscopic DNA biosensors based on different nanoparticles, sequential and simultaneous analysis, detection of carcinogen compounds and DNA damage
Biography
Biography: Ali A Ensafi
Abstract
DNA is an important functional biomolecule, which has received considerable attention by researchers. The detection of DNA damage has become one of the most important themes in DNA research fields because of the crucial role of DNA in mutagenesis, carcinogenesis and aging. If the damaged DNA cannot be repaired properly, the caused gene mutation will result in cancer and tumor in DNA replication process. DNA electrochemical sensors are most likely to become the accurate, sensitive, and rapid detection method for the transgenic plant products. Drugs that intercalate into ds-DNA have been extensively studied, and the field has been recently reviewed using variety techniques. Different types of supports have been used to prepare highly sensitive and selective electrochemical and optical biosensors to detect DNA damage and/or to quantify some pharmaceuticals or chemical compounds at trace levels, using voltammetry, electrochemical impedance spectroscopy and fluorescence method. Moreover, several nanocompounds have been used to fabricate DNA biosensors, including carbon nanotubes, carbon nanotubes modified with chitosan, carbon nanotubes modified with highly positive organic compounds, carbon nanotubes modified with TiO2 nanoparticles, graphene modified with some nanoparticles and/or amino acid-modified clay. Moreover, single layer and/or layer-by-layer methods have been used to fabricate the biosensors too. As a probe or detection system, several methods have been used including monitoring the oxidation peaks of guanine and adenine, reduction or oxidation peaks of some organic molecules (when intercalate with DNA, such as methylene blue, acridine orange) as a probe at the biosensors surface and/or the impedance spectra of the biosensor.