Bhanu Neupane
Kathmandu Institute of Applied Sciences, Nepal
Title: Design of stimulated emission depletion microscope and applications on cell imaging
Biography
Biography: Bhanu Neupane
Abstract
Stimulated Emission Depletion (STED) microscopy is a powerful optical imaging tool that provides diffraction unlimited resolution down to few tenth of nanometers in cell and tissue samples. STED microscope can be built in different versions. In this paper, detail design of continuous wave STED microscope will be discussed. Such microscope shows excellent performance in resolving self assembly patterns of polymeric nanoparticles at various ionic strength conditions. The application of this microscope in resolving nanoscopic cytoskeleton protein structures in both fixed and live cells will be presented. Other potential applications of such microscope in cellular and tissue imaging will be discussed.