Mark Bradley
University of Edinburgh, UK
Title: Optimal reporters for electrochemical detection of protease activity
Biography
Biography: Mark Bradley
Abstract
Electrochemical, peptide-based, biosensors are attracting significant attention for the detection and analysis of proteins. Among them, proteases are interesting targets due to their relevance in many pathophysiological conditions, including inflammation and cancer. Different platforms have been suggested for the electrochemical detection of these enzymes, as they catalyze the cleavage of amide bonds at specific sites in a protein or peptide. To meet the increasing demand for selective and sensitive analytical tools for the detection of proteases, improved analytical features, such as reduced limits of detection and sensor stability, are important. Here we investigate the effect of the nature and length of a spacer on the sensitivity of the electrochemical sensor. Redox-tagged peptides, tethered to a gold surface through a self-assembled monolayer (SAM), were selected as the Biosensing platform for the electrochemical detection of protease activity.
