Serban F Peteu
Michigan State University, USA
Title: Peroxynitrite detection: Recent innovations and remaining challenges
Biography
Biography: Serban F Peteu
Abstract
The most harmful of the reactive nitrogen and oxygen species in vivo is peroxynitrite (ONOO—), the reaction product of nitric oxide and superoxide. When present in excess, peroxynitrite is known to disturb the physiological balance in organisms leading to the nitro-oxidative stress. Its extended action over time through nitro-oxidative reactions in vivo is clinically correlated with chronic degenerative diseases, such as: cardiac (myocarditis; graft rejection; heart failure), vascular (atherosclerosis; aging; hypertension); neurodegenerative (Parkinson’s; Alzheimer’s; multiple sclerosis); diabetes and complications (neuropathy; nephropathy; retinopathy) and inflammation (chronic; toxic origin; arthritis). The ability of biosensors for rapid and real-time analysis in a multi-analyte format at relatively low cost will allow the quantitative and qualitative detection of ONOO—. The peroxynitrite sensitive and selective sensors and probes will facilitate a screening-type analysis and potentially prevent these numerous diseases. Consequently, many efforts are underway to detect peroxynitrite in biological media. This invited review will critically discuss for the first time the very latest innovations in the field of peroxynitrite biosensors and probes for in vivo and in vitro studies with the remaining challenges. Thus, the main trends will be extracted, in order to chart the future directions and hence create an instrumental outlook.