A P Vladimirov
Ural Federal University, Russia
Title: The use of the speckle dynamics for the quantitative analysis of micro and macro processes in cultured cells: Theory and experiment
Biography
Biography: A P Vladimirov
Abstract
Previously, the theory of dynamic speckle-interferometry which allows to study intracellular phenomena was developed by the author. The theory has been successfully used by the author with colleagues to study the effects of herpes virus in three types of cultured cells. The purpose of this report is to familiarize colleagues with the specificities of the theory and application of the method. First briefly discusses the theory of the method, the basic formulae used in practice. It is shown that the time-average intensity at the observation point and the correlation coefficient of the area of images depend on the parameters characterizing macroscopic changes (shape of cells) and microscopic processes in cells. The experiment with thawed cells, where the shape changes of cells due to the movement and the passing of intracellular processes is discussed.
