Dawid Nidzworski
SensDx Ltd, Poland
Title: New ultrasensitive BDD-biosensor for influenza virus detection
Biography
Biography: Dawid Nidzworski
Abstract
Influenza is a contagious disease caught by humans and caused by viruses belonging to the family Orthomyxoviridae. Each year, the influenza virus infects millions of people and kills hundreds of thousands of them. Economic losses caused by employee absenteeism are counted in the hundreds of millions of dollars a year. In order to successfully treat influenza virus infections, it is necessary to detect virus during initial development phase of the infection when tens to hundreds of viruses are present in pharynx of the patient. Here, we show a new universal diamond biosensor which enables detection of the virus at ultralow concentration even before clinical symptoms. A diamond electrode is modified with polyclonal anti-M1 antibodies and then a universal biomarker of influenza virus – M1 protein can be captured. In this assay, we observe a change in electrochemical impedance spectra. A detection limit of 1×10-14 g/mL in saliva buffer is achieved with M1 biomarker corresponds with 5-10 virus particles in samples. Also the universality of the assay was confirmed analyzing different strains of influenza A virus.