Yong Shin
Asan Medical Center, South Korea
Title: Rapid bio-optical sensors for molecular diagnostic in human diseases
Biography
Biography: Yong Shin
Abstract
Rapid, early, and accurate diagnosis of human diseases including human cancers, infectious diseases is essential for effective disease management and surveillance, and can reduce morbidity and mortality associated with the disease. Although significant advances have been achieved for the diagnosis of human diseases, these technologies are still far from ideal, being time consuming, complex and poorly sensitive and specific for clinical use as well as requiring separate assays for sample processing and detection. Recently, my team reports an isothermal, label-free, one-step DNA or RNA amplification and detection system, termed as ISAD for DNA and iROAD for RNA, for the diagnosis of human diseases. It couples one-step isothermal nucleic acid amplification method and bio-optical sensor based on silicon micro-ring resonator for simultaneous DNA or RNA amplification/detection in a label-free and real-time manner. The bio-optical sensor assay offers a one-step amplification/detection example to rapid analysis (< 20 min). The detection limit of the bio-optical sensor assay was found to be 10-times more sensitive than that of conventional methods including PCR and real-time PCR. We confirmed the clinical utility of the bio-optical sensor by detecting several targets (DNA or RNA) obtained from several human disease samples, such as tuberculosis, respiratory virus, malaria, and human cancers. We envision that the bio-optical sensor assay will be useful and potentially adaptable for better diagnosis of diverse human diseases including emerging infectious diseases, cancer, and neurological disorders.