5^th Euro Biosensors & Bioelectronics Conference

Biography

A biosensor is a promising device, which is combination of sensitivity of electrochemistry and specificity of biological recognition, enables to detect any kind of molecules in a short time with selectively and sensitively. Likewise, many analytical methods, it has also limitations, such as high oxidation potentials lead to detection of non-target molecules, furthermore non-electroactive species cannot show electroactive signal for measurement or some biomolecules cannot be transformed by enzymes, even if they can be transformed, they require secondary molecules such as mediators, coenzymes or labels. In order to detect molecules without electrochemical reaction, electrochemical impedance spectroscopy (EIS) can be employed as a measurement technique “to see electrode surface modifications just by looking impedance curves”. As it is known, electrochemical impedance spectroscopy is an electrochemical technique that provides the examination of electrical properties of electrode surface and binding kinetics of molecules between electrolyte and electrode surface. Therefore, it can be used for biomolecular recognition, biomolecular bindings and biomolecular interactions between molecules such as DNA-DNA, DNA-protein, Receptor-Ligand, Protein-Ligand, Antibody-Antigen, and Ion Channels-Ligands. As a consequence of this affinity provides label-free detection without chemical transformation and this binding property can be monitored by electrochemical impedance spectroscopy expeditiously.

Abstract

Abstract : Impedimetric (bio)sensors for label-free detection