Anupama Aili is pursuing her Masters degree in Electrical Engineerig at the University of Texas at arlington. Sheis currently doing her thesis in MEMS and Biomedical sensing aiming to integrate the Nano sensors in the medical sensing technology.
Microelectromechanical systems (MEMS) has provided the emergence of a new generation of sensor platforms. The operation of Acoustic sensor in the native environment of biomolecules as well as in liquid, causes, however, prominent loss of sensing performance. The loss factors being viscous drag and rely on the availability of capture molecules to bind analytes of interest to the sensor surface. So in order to avoid the current problem, here we present a strategy to interface MEMS sensors and the microfluidic platforms by an aerosol spray. By dry mass-sensing technique, the results demonstrate that the standard resolution limits of cantilever sensing in dynamic mode can be overcome with the integration of spray microfluidics with MEMS.
Maryam Imani has completed her BSc in genetic at the age of 21 years from shahid bahonar university (2013). She is working as genetic counsellor in satate of welfare organization of IRAN since 2014. She is studying MSc of biophysic at alzahra university. Interested field of research is: Genetic, molecular sensing, DNA biosensor, nanotechnology and MEMs.
Magnetic nano particles(MNPs), are widely studied because of their applications in various fields of biology and medicine such as magnetic bio-separation, detection of biological entities(nucleic acids, cells, protein) , clinic diagnosis and biological labels.For implemented application of MNPs, particles must have combined properties of high magnetic saturation, stability, biocompatibility and interactive functions at the surface.Using multiple linkers for Single Stranded DNA (ssDNA)immobilization on MNPs, can kept the DNA away from the surface of MNPs, which is valuable for the promotion of the hybridization efficiency.In this study Silane agent(APTES) was chosen for modifying the surface of MNPs directly, because of its advantages including :biocompatibility, high saturation magnetization as well as high density of surface functional end group that allows for connecting to other linker. Second linker is Terephthalaldehyde(TPA); a dialdehydereagent that has high reaction efficiency and riskless by product H2O. TPA with a rigid phenyl group shows less flexibility compared to other common linkers used for DNA attachment,which makes it more efficient cross-linker to graft the biomolecules . In our study, MNPs were prepared by coprecipitation methods and then, APTES and TPA were coated on the surface of MNPs respectively. Finally, aldehyde group of TPA was used for capturing amino modified ssDNA via schiff base formation. Two linkers made enough space for more efficient DNA hybridization and MNPs kept their good saturation magnetization. FTIR spectra indicated that all of the reagent(APTES,TPA,ssDNA) have been bound on the surface of MNPssuccessfully. The attachment of fluorescently labeled ssDNA in this study was confirmed by fluorescent microscopy. The amount of ssDNA loaded on Fe3O4 nanoparticles was quantitatively evaluated.VSM and FE SEM analysis were performed to evaluate saturation magnetization and particle size.