10^th International Conference & Exhibition on Biosensors & Bioelectronics September 21-22, 2018 | Dallas, Texas, USA

Anupama Aili is pursuing her Masters degree in Electrical Engineerig at the University of Texas at arlington. Sheis currently doing her thesis in MEMS and Biomedical sensing aiming to integrate the Nano sensors in the medical sensing technology.

Microelectromechanical systems (MEMS) has provided the emergence of a new generation of sensor platforms. The operation of Acoustic sensor in the native environment of biomolecules as well as in liquid, causes, however, prominent loss of sensing performance. The loss factors being viscous drag and rely on the availability of capture molecules to bind analytes of interest to the sensor surface. So in order to avoid the current problem, here we present a strategy to interface MEMS sensors and the microfluidic platforms by an aerosol spray. By dry mass-sensing technique, the results demonstrate that the standard resolution limits of cantilever sensing in dynamic mode can be overcome with the integration of spray microfluidics with MEMS.

Maryam Imani has completed her BSc in genetic at the age of 21 years from shahid bahonar university (2013). She is working as genetic counsellor in satate of welfare organization of IRAN since 2014. She is studying MSc of biophysic at alzahra university. Interested field of research is: Genetic, molecular sensing, DNA biosensor, nanotechnology and MEMs.

Magnetic nano particles(MNPs), are widely studied because of their applications in various fields of biology and medicine such as magnetic bio-separation, detection of biological entities(nucleic acids, cells, protein) , clinic diagnosis and biological labels.For implemented application of MNPs, particles must have combined properties of high magnetic saturation, stability, biocompatibility and interactive functions at the surface.Using multiple linkers for Single Stranded DNA (ssDNA)immobilization on MNPs, can kept the DNA away from the surface of MNPs, which is valuable for the promotion of the hybridization efficiency.In this study Silane agent(APTES) was chosen for modifying the surface of MNPs directly, because of its advantages including :biocompatibility, high saturation magnetization as well as high density of surface functional end group that allows for connecting to other linker. Second linker is Terephthalaldehyde(TPA); a dialdehydereagent that has high reaction efficiency and riskless by product H2O. TPA with a rigid phenyl group shows less flexibility compared to other common linkers used for DNA attachment,which makes it more efficient cross-linker to graft the biomolecules . In our study, MNPs were prepared by coprecipitation methods and then, APTES and TPA were coated on the surface of MNPs respectively. Finally, aldehyde group of TPA was used for capturing amino modified ssDNA via schiff base formation. Two linkers made enough space for more efficient DNA hybridization and MNPs kept their good saturation magnetization. FTIR spectra indicated that all of the reagent(APTES,TPA,ssDNA) have been bound on the surface of MNPssuccessfully. The attachment of fluorescently labeled ssDNA in this study was confirmed by fluorescent microscopy. The amount of ssDNA loaded on Fe3O4 nanoparticles was quantitatively evaluated.VSM and FE SEM analysis were performed to evaluate saturation magnetization and particle size.

Barhoumi Lassaad is a PhD student in NANOmaeterials and Microsystems for Health care, Envirenement Monitoring and Energy (NANOMISENE) Laboratory, Centre for Research on Microelectronics and Nanotechnology CRMN, Technopark of Sousse, University of Sousse. He is engaged in the characterization of metal nanostructures and its application in sensing.

We report in this work, the synthesis and characterization of a novel immunosensor based a screen-printed gold electrode (SPAuE) modified with a new structure of iron magnetic nanoparticles coated with poly (pyrrole-co-pyrrole-2-carboxylic acid, Py-Py-COOH) (Py/Py-COOH/MNP) [1] particles to increase the immunosensor sensitivity of Tumor Necrosis Factor-α (TNF-α) [2, 3]. TNF-α antibodies were covalently bonded to Py/Py-COOH/MNP modified SPEAu. A sandwich-type detection strategy was then employed for antigen (Ag-TNF-α) detection through the labeled conjugate antibody (Ab-TNF-α-HRP) activity in a TMB solution. Finally, the chronoamperometry technique was applied to characterize the modified SPEAu. The use of a conjugate antibody anti-TNF-α labeled with horseradish peroxidase (Ab-TNF-α-HRP) was investigated using tetramethylbenzidine (TMB) substrate as electrochemical substrate. The modified screen-printed gold electrode (SPEAu) was characterized for the first time, using atomic force microscopic (AFM) and scanning electron microscopy (SEM). The specificity of the immune-sensor was then investigated under the optimal experimental conditions by analyzing aqueous solutions containing possible interferences represented by other salivary cytokines secreted in the acute stage of inflammation, such as interleukin-6 (IL-6) and interleukin-10 (IL-10). The developed immunosensor showed good performances for Ag-TNF-α detection within the range of 1 pg mL-1 to 15 pg mL- 1 of antigen TNF-α was determined at 1 pg mL-1. The present immune-sensor is this very promising for sensitive and rapid detection of antigen Ag-TNF-α in clinical sample

Darkwah Williams Kwekuis studying his Masters in Environmental Engineering at Hohai University. He has published more than 3 papers in reputed journals.

Ridwan Fayaz Hossain is a Ph.D. candidate in Electrical Engineering department at University of North Texas. His Ph.D. research is focused on ink-jet printing of 2D layered materials for flexible and printed electronics, with a particular emphasis on bio-related applications and bioelectronics. He has published more than 15 papers in reputed journals (Nature 2D materials and Application, 2D Materials, Journal of Materials Chemistry C, Biomedical Microdevices etc.).

Age-related macular degeneration (AMD), a retinal degenerative disease that results in a continuous degeneration of photoreceptors in the retina which eventually leads to complete blindness. One approach to combat AMD is through the use of artificially implantable photodetectors that are physically placed on the retina. The large format photodetector pixels on the flexible and conformable substrate allows the implantable photodetectors to be in intimate contact to retinal pigment epithelium. Interestingly, 2D materials such as photosensitive and semiconducting molybdenum disulfide (MoS2) and electrically conducting graphene have recently received tremendous promise due to their unique photonic and optoelectronic properties properties and their potential in various types of micro and nano devices. In this study, we have tested the biocompatibility of various 2D materials, such as graphene and MoS2 in several organic solvents. Specifically, these materials have been dispersed in Isopropyl Alcohol (IPA), a mixture of Cyclohexanone/Terpineol 7:3 ratio (C/T), and N-Methyl-2-pyrrolidone (NMP). Mouse Embryonic Fibroblast (STO) was used for the biocompatibility analysis for inks drop cast on flexible polyimide substrate. The inks formed using 2D graphene and MoS2, were highly biocompatible on polyimide substrates, where a cell survival rate of up to 98% was measured for the STO, while the cell confluence rate was in between 70-98%. Here, a new approach was utilized to form photosensitive pixels that utilize heterostructures of inkjet printed MoS2 and graphene, using inks that also show a high degree of biocompatibility. The inkjet printed 2D heterostructure devices were photoresponsive to broadband incoming radiation in the visible regime, and the photocurrent scaled proportionally with the incident light intensity, exhibiting a photoresponsivity R ~ 0.30 A/W. This is 103 times higher compared to prior reports, and detectivity D was calculated to be ~ 3.6 × 1010 Jones at room temperature. Strain-dependent measurements of photocurrent with bending was also conducted, that showed a photocurrent of ~ 1.16 µA with strain levels for curvature up to ~ 0.262 cm-1, indicating the feasibility of such devices for large format arrays printed on flexible substrate, unlike conventional Si implantable detectors that are rigid and nonconformable. In conclusion, the inkjet printed, biocompatible 2D hetero-junction photodetector formed on flexible and conformable substrates was successfully shown to be photoresponsive to a wide range of light intensities and strain levels, making it a promising prospect for in vivo bio-sensing applications for AMD.