Greter Amelia Ortega RodrÃguez
Center for Applied Science and Advanced Technology of IPN, Mexico
Title: Magnetite nanoparticles on paper: A platform for the diagnosis of dengue fever by magnetic -ELISA
Biography
Biography: Greter Amelia Ortega RodrÃguez
Abstract
Enzyme-linked immunosorbent assays (ELISAs) are the most widely used methods to detect antibodies. However, they have some drawbacks. As a result, in recent years magnetic nanoparticles or beads (e.g. magnetites) have been combined with ELISAs to improve their analytical performance. On the other hand, despite paper-based ELISA are less sensitive than conventional ELISA, they emerge as suitable platforms to develop disposable devices for point-of-care diagnostic. A novel “magnetic-ELISA”, based on core-shell magnetite@polydopamine nanoparticles supported on Whatman paper was developed to detect IgM-dengue antibodies. An affordable procedure to deposit magnetite nanoparticles on paper surfaces (Whatman thype-1 and Whatman thype-ss903) and, to conjugate such nanoparticles with Anti human-IgM antibodies using polydopamine as linker, is reported. Structural features, magnetic behavior, coating homogeneity, as well as, the nanoparticles/linked antibodies ratio were determined. The analytical performance of “magnetic-ELISA” supported on paper surface was 100 times more sensitive with a 700 times lower limit of detection than traditional ELISA or using magnetic beads without depositing on paper to detect IgM-dengue antibodies. Additionally, the new system showed low background, acceptable reproducibility, low-cost, easy manufacturing and effortless and easy handling which are very important, considering the large number of biological samples to be processed by a laboratory in case of dengue epidemics.