M Carmen Bermudo Redondo
Rovira i Virgili University, Spain
Title: Impedimetric electrochemical sensor for albumin determination based on poly bromocresol purple film modified electrodes
Biography
Biography: M Carmen Bermudo Redondo
Abstract
Human serum albumin (HSA) is the major protein in plasma synthesized principally in the liver and playing significant roles after it is released in the circulation. Albumin is a very important factor of regulation in the exchange of water between the plasma and the interstitial compartment being largely responsible of the colloidal osmotic pressure of blood. Moreover, it is a protein with a notable ability to bind an extensive range of other small molecules helping in the transport of important substances in the human body such as hormones, fatty acids and drugs. A decrease of albumin level in serum is associated with severe illnesses of the kidney as well as other conditions such as liver disease, malnutrition and extensive burns. Therefore, HSA determination is extremely useful in the diagnosis and treatment of many clinical entities. This research work reports on the development and integration of a novel label-free impedimetric sensor based on a poly (bromocresol purple) surface for the specific detection of HSA. The fabricated sensor was incubated with serum albumin, and electrochemical impedance spectroscopy (EIS) was employed to measure the changes in the conductance of the electrode of bromocresol purple (BCP) by reacting with the albumin. In addition, we validated the specificity of the designed sensor to only albumin. Clinical applicability of the sensor was also demonstrated utilizing real serum sample from patients obtaining excellent agreement with the commercial available colorimetric kit. It is expected that the new poly (BCP) sensor will become a successful diagnostic platform for HSA detection in clinical diseases.