DNA Chips and Nucleic acid Sensors

Aptamers are oligonucleotide or peptide molecules that bind to a specific target molecule. Aptamers are usually created by selecting them from a large random sequence pool, but natural aptamers also exist inriboswitches. Immunosensors are built by means of the appropriate combination of the biomolecules with the transducer used together, they can be applied  in specific analytical situations. Immunosensors commonly rely on the reuse of the same receptor surface for many measurements. A Biochip is a combination of minute DNA spots hooked up to a hard surface. Scientists use DNA Biochips to check the expression levels of huge number of genes at the same time. Each DNA spot contains picomoles of a precised DNA sequence known as a probe. These can be tiny section of a gene or a DNA particle that are used to cross breed a cDNA or cRNA. Probe-target cross breeding is usually quantified and detected by detection of fluorophore. Silver or chemiluminescence-labeled targets to identify corresponding abundance of nucleic acid sequences in the target. Sensors are devices that respond to physical or chemical stimuli and produce detectable signals. They are a critical extension of human perception of the world in many aspects of the modern society. This is largely because we are much less sensitive to the chemical or biological environment than to the physical environment (e.g., light, pressure, temperature, or humidity). However, appropriate chemical or biological compositions are tightly linked to the quality of life.

  • Aptamers and their biological applications
  • Proteomics, single cell analysis, and electronic noses
  • Immunosensors
  • Natural & synthetic receptors (including Molecularly imprinted polymers)
  • Organism and whole cell-based biosensors

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