Enzyme-Based Chemical Biosensors


Enzyme-based biosensors, using enzymes as the recognition elements and combining the inherent specificity of enzymes with the particular advantages of biosensors, have been considerably applied in numerous fields. Enzyme-based chemical biosensors are primarily based on biological recognition. In order to operate, the enzymes need to be available to catalyze a specific biochemical reaction and be secure under the normal operating conditions of the biosensor.Analytes such as lactate, glucose, glutamate, and glutamine play a vital role in the metabolism of living organisms. Glutamine and glucose support the cell function and growth; lactate is developed by cells and allows judging the efficiency of the cells metabolism; and glutamate is an amino acid consumed by cells. Each of these analytes needs a particular set of enzymes for its detection.


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